Development of fluorinated pseudo-cyclic phospholipids as membrane protein reconstitution matrices

2014 Fiscal Year Final Research Report

• Project/Area Number: 23590039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical pharmacy
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TAKAGI TOSHIYUKI 独立行政法人産業技術総合研究所, 幹細胞工学研究センター, 主任研究員 (10248065)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: フッ素 / 人工脂質 / 膜タンパク質 / ベシクル / 擬環状脂質 / バクテリオロドプシン

Outline of Final Research Achievements

Fluorinated compounds exhibit unique properties such as thermal and chemical stability, remarkable hydrophobicity and lipophobicity. Sufficiently long perfluoroalkyl segments are strongly hydrophobic and more rigid than hydrocarbon ones, leading to the formation of mechanically stable and rigid assemblies, of which properties are not preferable for membrane protein reconstitution matrices. The introduction of a partially fluorinated alkyl group into a pseudo-cyclic phospholipid is considered much effective to provide and/or control the suitable fluidity for their monolayer, which is essential for membrane protein reconstitution techniques.

Free Research Field

有機フッ素化学