2013 Fiscal Year Final Research Report
Development of pH-responsive bio-device by utilizing homomeric to heteromeric coiled-coil conversion
| Project/Area Number |
23590058
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Meijo University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | バイオセンサー / コイルドコイル / FRET / ｐH / 輸送タンパク質 |
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| Research Abstract |
In this study, we demonstrated that the coiled-coil domains isolated from the yeast cargo receptors Emp46p/47p show pH-dependent conversion of heteromeric to homomeric complexes, and identified the amino acid residues responsible for the regulation of the conversion. Mutations introduced at these residues altered the pH-dependency of the formation of heteromeric complexes. Moreover, using fluorescent protein fusions, the assembly of heteromeric complexes was successfully detected by FRET, which enabled real-time monitoring of the heteromeric complex formation. Thus, we have succeeded in providing strategies for application of the coiled-coil domains of Emp46p/47p to various pH-responsive bio-devices.
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Research Products
(5 results)
