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2013 Fiscal Year Final Research Report

Elucidation of the physiological function of iNOS degradation system by generating mice in which the SPSB inhibitor is expressed throughout the body

Research Project

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Project/Area Number 23590066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionOkayama University

Principal Investigator

NISHIYA Tadashi  岡山大学, 医歯(薬)学総合研究科, 准教授 (80399831)

Project Period (FY) 2011 – 2013
KeywordsSPSB / iNOS / CDC14A / FOG-2 / CTTNBP2 / E3 / ユビキチン化 / 蛋白質分解
Research Abstract

We attempted to generate the mice expressing SPSB inhibitor which blocks the association between SPSB and its substrates. Although several F1 mice in which the SPSB inhibitor-expressing gene was introduced were obtained, SPSB inhibitor was not detected in kidneys and livers from these mice. In parallel to this experiment, we identified CDC14A, CTTNBP2 and FOG-2 as novel substrates for SPSB. These substrates are involved in wide variety of physiological functions such as cell cycle control, development of heart and lung, and the dendritic spine formation. Therefore, the inhibition of the degradation of these substrates might cause serious problems for mouse development.

  • Research Products

    (12 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results)

  • [Journal Article] Nicotine- and Tar-Free Cigarette Smoke induces cell damage through reactive oxygen species newly generated by PKC-dependent activation of NADPH oxidase2012

    • Author(s)
      Asano H, Horinouchi T, Mai Y, Sawada O, Fujii S, Nishiya T, Minami M, Katayama T, Iwanaga T, Terada K, Miwa S.
    • Journal Title

      J. Phar macol. Sci

      Volume: 118(2) Pages: 275-287

    • Peer Reviewed
  • [Journal Article] Adenylate cyclase-cAMP-protein kinase A signaling pathway inhibits endothelin type A receptor-operated Ca^<2+> entry mediated via TRPC6 channels2012

    • Author(s)
      Horinouchi T, Higa T, Aoyagi H, Nishiya T, Terada K, Miwa S.
    • Journal Title

      J. Phar macol. Exp. Ther

      Volume: 340(1) Pages: 143-151

    • Peer Reviewed
  • [Journal Article] Function and regulation of endothelin type A receptor-operated transient receptor potential canonical channels2011

    • Author(s)
      Horinouchi T, Terada K, Higa T, Aoyagi H, Nishiya T, Suzuki H, Miwa S.
    • Journal Title

      J . Phar macol. Sci

      Volume: 117(4) Pages: 295-306

    • Peer Reviewed
  • [Journal Article] On-off system for PI3-kinase-Akt signaling through S-nitrosylation of PTEN2011

    • Author(s)
      Numajiri N, Takasawa K, Nishiya T, Tanaka H, Ohno K, Hayakawa W, Asada M, Matsuda H, Azumi K, Kamata H, Nakamura T, Hara H, Minami M, Lipton S, Uehara T.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 108(25)(*Equal contribution) Pages: 10349-10354

    • Peer Reviewed
  • [Journal Article] NKG2D^+IFN-^+CD8^+T cells are responsible for palladium allergy

    • Author(s)
      Kawano M, Nakayama M, Aoshima Y, Nakamura K, Ono M, Nishiya T, Nakamura S, Takeda Y, Dobashi A, Takahashi A, Endo M, Ito A, Ueda K, Sato N, Higuchi S, Kondo T, Hashimoto S, Watanabe M, Watanabe M, Takahashi T, Sasaki K, Nakamura M, Sasazuki T, Narushima T, Suzuki R, Ogasawara K.
    • Journal Title

      PLoS One

      Volume: 9(2) Pages: e86810

    • Peer Reviewed
  • [Presentation] 新規E3ユビキチンリガーゼECS(SPSB)の基質同定2014

    • Author(s)
      西屋禎、丸山拳、高澤孔明、浜田恭平、上原孝
    • Organizer
      第134回日本薬学会年会
    • Place of Presentation
      熊本大学、熊本市
    • Year and Date
      2014-03-30
  • [Presentation] E3ユビキチンリガーゼECS(SPSB)の新規基質の網羅的探索2014

    • Author(s)
      西屋禎、丸山拳、高澤孔明、浜田恭平、上原孝
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台国際センター、仙台市
    • Year and Date
      2014-03-19
  • [Presentation] E3ユビキチンリガーゼ"ECS(SPSB)"の新規基質の網羅的同定2013

    • Author(s)
      西屋禎、丸山拳、高澤孔明、浜田恭平、松本一馬、小笠原康悦、三輪聡一、上原孝
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸市
    • Year and Date
      2013-12-05
  • [Presentation] SPSBファミリー蛋白質を介したCDC14A phosphataseのユビキチン-プロテアソーム依存的分解機構2013

    • Author(s)
      西屋禎、丸山拳、松本一馬、小笠原康悦、三輪聡一、上原孝
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2013-03-23
  • [Presentation] CDC14AはElongin B/C-Cul5-SPSB型E3ユビキチンリガーゼの基質である2012

    • Author(s)
      西屋禎、松本一馬、小笠原康悦、三輪聡一、上原孝
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡、福岡市
    • Year and Date
      2012-12-16
  • [Presentation] 誘導型NO合成酵素の分解制御機構2011

    • Author(s)
      西屋禎、前川聡、松本一馬、堀之内孝広、藤室雅弘、小笠原康悦、上原孝、三輪聡一
    • Organizer
      第11回日本NO学会
    • Place of Presentation
      昭和薬科大学、東京
    • Year and Date
      20110513-14
  • [Presentation] 誘導型NO合成酵素のユビキチン・プロテアソーム依存的分解制御機構2011

    • Author(s)
      松本一馬、西屋禎、前川聡、堀之内孝広、藤室雅弘、小笠原康悦、上原孝、三輪聡一
    • Organizer
      第25回北海道薬物作用談話会
    • Place of Presentation
      酪農学園大学中央館学生ホール、江別市
    • Year and Date
      2011-07-30

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Published: 2015-07-16  

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