2013 Fiscal Year Final Research Report
Analysis of peroxisome membrane biogenesis and application for Nano-medicine
| Project/Area Number |
23590072
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | University of Toyama |
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Principal Investigator |
IMANAKA Tsuneo 富山大学, 大学院医学薬学研究部(薬学), 教授 (50119559)
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| Co-Investigator(Kenkyū-buntansha) |
KASHIWAYAMA Yoshinori 富山大学, 大学院医学薬学研究部(薬学), 助教 (20401812)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ペルオキシソーム / グリコソーム / ペルオキシソーム膜形成 / 膜タンパク質局在化 / トリパノソーマ感染症治療薬 / ハイスループットスクリーニング |
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| Research Abstract |
Pex3p, Pex19p and Pex5p, Pex14p are essential for the biogenesis of peroxisome. Putative Pex3p binding protein is suggested to regulate insertion of peroxisomal membrane proteins into the peroxisome. To identify the binding protein, we established yeast two-hybrid system using human cDNA library and found ten putative proteins.
Trypanosome has glycosome that resembles to human peroxisome. Impaired biogenesis of glycosome is known to show fatal effect for trypanosome. As an application of the knowledge about biogenesis of peroxisome, we tried to develop the therapeutic compounds for trypanosomiasis. We established a high thoughput screening system to identify chemical compounds that inhibit the interaction between Pex5p and Pex14p. Using this screening system, we started to screen chemical libraries to find effective compounds.
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[Journal Article] Molecular species of phospholipids with very long chain fatty acids in skin fibroblasts of Zellweger syndrome2013
Author(s)
Hama K., Nagai T., Nishizawa C., Ikeda K., Morita M., Satoh N., Nakanishi, H., Imanaka, T., Shimozawa N., Taguchi R., Inoue K., and Yokoyama K
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Journal Title
Lipids
Volume: 48
Pages: 1253-1267
Peer Reviewed
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