2013 Fiscal Year Final Research Report
Vesicle trafficking in phosphoinositide phosphatase-knockdown cells
| Project/Area Number |
23590078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
HAZEKI Osamu 広島大学, 医歯薬保健学研究院, 教授 (80142751)
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| Project Period (FY) |
2011 – 2013
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| Keywords | phosphoinositide / 3'-phosphatase / 4'-phosphatase / 5'-phosphatase / RAW264.7 / macrophages / shRNA / phagocytosis |
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| Research Abstract |
A series of RAW264.7 macrophages was cloned that stably express the shRNA targeting the specific one of the phosphoinositide phosphatase isozymes. The targeted enzymes were eight 3'-phosphatases (PTEN, Synaptojanin2, Sac1, Sac3, MTM1, MTMR2, MTMR9, MTMR14), one 4'-phosphatase (Inpp4a), and six 5'-phosphatases (SHIP1, SHIP2, Inpp5e, SKIP, OCRL, Inpp5b). Two lines of cells that express shRNA with different targeting sequence were prepared for each isozyme. The knockdown cells were examined for their ability to engulf IgG-opsonized particles. The amount and localization of phosphoinositide derivatives, PtdIns(3,4,5)P3, PtdIns(3,4)P2, and PtdIns(3)P, during the phagosome formation and maturation were also monitored. The results suggested several specific functions of the phosphatase isozymes.
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Research Products
(13 results)
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[Journal Article] Phosphoinositide 3-kinaseγcontrols the intracellular localization of CpG to limit DNA-PKcsdependent IL-10 production in macrophages2011
Author(s)
Hazeki K, Kametani Y, Murakami H, Uehara M, Ishikawa Y, Nigorikawa K, Takasuga S, Sasaki T, Seya T, Matsumoto M, Hazeki O
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Journal Title
PLoS ONE
Volume: 6
Pages: e26836
Peer Reviewed
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