2013 Fiscal Year Final Research Report
Lipopolysaccharide remodeling in bacterial adaptation to host
| Project/Area Number |
23590095
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Doshisha Women's College of Liberal Arts |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | エンドトキシン / Toll-like receptor / 細菌 / 外膜 |
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| Research Abstract |
Lipid A deacylated by outer membrane enzyme LpxR is less active than unmodified lipid A, but more active than lipid A precursor purified from LpxM mutant. These results suggest that lipid A modification by LpxR is beneficial for bacterial adaptation to host.
Outer membrane vesicle was prepared from E. coli W3110, and the structure of lipid A and its protein composition were examined. Specific lipid A modification was not observed. On the other hand, it was revealed that the outer membrane vescicle contained flagellin, a component of flagella. The ability to form flagella, including the synthesis of flagella proteins, affected the production of outer membrane vesicles.
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