2013 Fiscal Year Final Research Report
Essential role of poly(ADP-ribosyl)ation in maintenance of spermatogonial stem cells
| Project/Area Number |
23590097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka Ohtani University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 精子幹細胞 / ポリ(ADP-リボース) / 多能性幹細胞 / 発生・分化 / 再生医療 |
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| Research Abstract |
Spermatogonial stem cells (SSCs) can proliferate in vitro in the presence of glial cell line-derived neurotrophic factor, a self-renewal factor for SSCs. These cultured SSCs are called germline stem (GS) cells. In previous study, we examined the effect of PARP inhibitor, inhibition of poly(ADP-ribosyl)ation, on GS cells. Inhibition of poly(ADP-ribosyl)ation suppressed cell proliferation of GS cells, and induced cell death. In order to identify the causative genes that is involved in the pathway leading to the suppression of cell proliferation, the gene expression and protein phosphorylation analyses were carried out. Some genes in p53 signaling pathway were up-regulated after addition of PARP inhibitors. We then investigated activation of p53 by detection of its phosphorylation site. The phosphorylation was increased after addition of PARP inhibitors. These results suggest that poly(ADP-ribosyl)ation regulates activity of p53 and thus p53 signaling pathway in GS cells.
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