2013 Fiscal Year Final Research Report
An elucidation of hierarchy of a biological clock and a rhythm adjustment drug by generating a novel clock function-deficient mouse
| Project/Area Number |
23590107
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 視交叉上核 / 概日時計 / 時計遺伝子 / 時差 / バソプレッシン / 遺伝子改変マウス |
|---|
| Research Abstract |
The endogenous circadian clock drives oscillations in physiology and behavior with a period of about 24 hours. When travelling rapidly across multiple time zones (jet lag), we become aware of the desynchrony between our internal clock and external light-dark cycle. Although jet lag is recognized as a chronobiological problem, its specific molecular and cellular mechanisms are poorly understood.
In this study, we show that circadian rhythms of locomotor activity, clock gene expression, and body temperature rapidly re-entrained to phase-shifted light-dark cycles in mice genetically deficient in V1a and V1b receptors (V1aV1bDKO). Real-time imaging of cellular rhythms in the suprachiasmatic nucleus (SCN) slices suggested that interneuronal communication mediated by V1a and V1b confers on the SCN an intrinsic resistance to external rhythm perturbation. Pharmacological blockade of V1a and V1b in the SCN of wild-type mice accelerated the speed of recovery from jet lag.
|
-
[Journal Article] Mice Genetically Deficient in Vasopressin V1a and V1b Receptors Are Resistant to Jet Lag2013
Author(s)
Yamaguchi Y, Suzuki T, Mizoro Y, Kori H, Okada K, Chen Y, Fustin JM, Yamazaki F, Mizuguchi N, Zhang J, Dong X, Tsujimoto G, Okuno Y, Doi M, Okamura H
-
Journal Title
Science
Volume: 342
Pages: 85-90
DOI
Peer Reviewed
-
-
-
-
[Journal Article] Circadian regulation of intracellular G-protein signalling mediates intercellular synchrony and rhythmicity in the suprachiasmatic nucleus2011
Author(s)
Doi M, Ishida A, Miyake A, Sato M, Komatsu R, Yamazaki F, Kimura I, Tsuchiya S, Kori H, Seo K, Yamaguchi Y, Matsuo M, Fustin JM, Tanaka R, Santo Y, Yamada H, Takahashi Y, Araki M, Nakao K, Aizawa S, Kobayashi M, Obrietan K, Tsujimoto G, Okamura H
-
Journal Title
Nature communications
Volume: 2
Pages: 327
DOI
Peer Reviewed
-
[Journal Article] Accurate Determination of S-Phase Fraction in Proliferative Cells by Dual Fluorescence and Peroxidase Immunohistochemistry with 5-Bromo-2'-Deoxyuridine (BrdU) and Ki67 Antibodies2011
Author(s)
Tanaka R, Tainaka M, Ota T, Mizuguchi N, Kato H, Urabe S, Chen Y, Fustin JM, Yamaguchi Y, Doi M, Hamada S, Okamura H
-
Journal Title
J Histochem Cytochem
Volume: 59
Pages: 791-798
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
