2013 Fiscal Year Final Research Report
Role of miRNAs in the development of bronchial smooth muscle hyperresponsiveness in allergic asthma
| Project/Area Number |
23590118
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Hiroyasu 星薬科大学, 薬学部, 講師 (00328923)
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| Project Period (FY) |
2011 – 2013
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| Keywords | アレルギー性気管支喘息 / 気道過敏性 / 気管支平滑筋 / microRNA (miRNA) / miR-140 / RhoA / CD38 / Interleukin-13 (IL-13) |
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| Research Abstract |
Current study was carried out to make clear the mechanism(s) of development of airway hyperresponsiveness in asthmatics. In bronchial smooth muscles of mice with allergic asthma, an up-regulation of RhoA protein and a down-regulation of miR-140-3p were observed. In cultured human bronchial smooth muscle cells, an up-regulation of RhoA protein was observed when the function of endogenous miR-140-3p was inhibited by its antagomir. Treatment of the cells with interleukin-13 caused an up-regulation of RhoA protein and a down-regulation of miR-140-3p. These findings suggest that RhoA protein expression is negatively regulated by miR-140-3p in bronchial smooth muscle cells. The increased expression of interleukin-13 in the airways of asthmatics might, at least in part, contribute to the reduction of miR-140-3p, resulting in an up-regulation of RhoA protein and augmented smooth muscle contraction of the airways.
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Research Products
(28 results)
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[Journal Article] The gene expression of aquaporins in various cutaneous tissues of the mouse2014
Author(s)
Sakai, H., Sato, K., Takase, K., Hirosaki, A., Jo, A., Sugiyama, R., Chiba, Y. and Narita, M
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Journal Title
J. Exp. Integr. Med
Volume: 4
Pages: 75-79
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[Journal Article] Involvement of the Tyr kinase/JNK pathway in carbachol-induced bronchial smooth muscle contraction in the rat2013
Author(s)
Sakai, H., Watanabe, Y., Honda, M., Tsuiki, R. Ueda, Y., Nagai, Y., Narita, M. and Chiba, Y
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Journal Title
Anesthesiology
Volume: 118
Pages: 1076-1085
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[Journal Article] Elevated guanylate cyclase and cyclic-guanosine monophosphate-dependent protein kinase levels in nasal mucosae of antigen-challenged rats2013
Author(s)
Sakai, H., Hara, T., Todoroki, K., Igarashi, Y., Misawa, M., Narita, M. and Chiba, Y
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Journal Title
Microvasc. Res
Volume: 90
Pages: 150-153
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[Journal Article] Effect of sugammadex on bronchial smooth muscle function in rats2012
Author(s)
Yoshioka, N., Hanazaki, M., Fujita, Y., Nakatsuka, H., Katayama, H. and Chiba, Y
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Journal Title
J. Smooth Muscle Res
Volume: 48
Pages: 59-64
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[Journal Article] Different effects of smoke from heavy and light cigarettes on induction of bronchial smooth muscle hyperresponsiveness in rats2011
Author(s)
Sakai, H., Fujita, A., Watanabe, A., Chiba, Y., Kamei, J. and Misawa, M
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Journal Title
J. Smooth Muscle Res
Volume: 47
Pages: 1-10
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[Journal Article] Mechanisms of cisplatin-induced muscle atrophy
Author(s)
Sakai, H., Sagara, A., Arakawa, K., Sugiyama, R., Hirosaki, A., Takase, K., Jo, A., Sato, K., Chiba, Y., Yamazaki, M., Matoba, M. and Narita, M
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Journal Title
Toxicol. Appl. Pharmacol
Volume: (in press)
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[Journal Article] Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
Author(s)
Sakai, H., Watanabe, A., Fujita, A., Misawa, M., Narita, M. and Chiba, Y
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Journal Title
J. Smooth Muscle Res
Volume: (in press)
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