Development of the malaria detection system that can be used for antimalarial drug screening

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: YATSUSHIRO shouki 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (90399155)
• Co-Investigator(Renkei-kenkyūsha): 山村 昌平 産総研, 主任研究員
• Project Period (FY): 2011 – 2013
• Keywords: マラリア原虫 / 細胞チップ / 薬剤スクリーニング

Research Abstract

We have developed a basic technology for a malaria diagnosis system using a cell microarray chip. The chip, with 20,944 microchambers (105 microm width and 50 microm depth) was made from polystyrene. About 130 erythrocytes were accommodated in each microchamber with monolayer formation, there being over 2,700,000 erythrocytes in total on a chip. This detection method promises the potential of a cell microarray chip for the early detection of malaria offering 100 times higher sensitivity than of light microscopy with Giemsa staining.
We are now developing the system based on cell microarray chip technology including erythrocytes isolation column and exclusive fluorescence detector in cooperation with electrical appliance manufacturer. We examined 17 blood samples which were obtained from malaria patients in Hospital using developing diagnosis system. Good correlation was observed between cell microarray chip method and conventional light microscopy.

Research Products

• [Journal Article] Simultaneous immunoassay analysis of plasma IL-6 and TNF-alpha on a microchip (2013)
  • Author(s): Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, Kido J, Tanaka M, Shinohara Y, Baba Y, Ooie T, Kataoka M
  • Journal Title: PLoS one Volume: 8(1) Pages: e53620
• [Journal Article] Accurate detection of carcinoma cells by use of a cell microarray chip (2012)
  • Author(s): Yamamura S, Yatsushiro S, Yamaguchi Y, Abe K, Shinohara Y, Tamiya E, Baba Y, Kataoka M
  • Journal Title: PLoS one Volume: 7(3) Pages: e32370
• [Journal Article] Quantitative analysis of serum procollagen type I C-terminal propeptide by immunoassay on microchip (2011)
  • Author(s): Yatsushiro S, Akamine R, Yamamura S, Hino M, Kajimoto K, Abe K, Abe H, Kido J, Tanaka M, Shinohara Y, Baba Y, Ooie T, Kataoka M
  • Journal Title: PLoS one Volume: 6(4) Pages: e18807
• [Presentation] Application of microchip electrophoresis for the rapid and accurate identification of Plasmodium falciparum substitute for secand PCR in nested PCR (2014)
  • Author(s): Obana E, Abe K, Yatsushiro S, Yamamura S, Kataoka M
  • Organizer: Advances in Biodetection & Biosensors 2014
  • Place of Presentation: ベルリン(ドイツ)
  • Year and Date: 20140300
• [Presentation] Recovery of malaria-infection erythrocyte from a cell microarray chip with a micomanipulator snd PCR analysis for species identification of malaria (2014)
  • Author(s): Abe K, Obana E, Yatsushiro S, Yamamura S, Kataoka M
  • Organizer: Advances in Biodetection & Biosensors 2014
  • Place of Presentation: ベルリン(ドイツ)
  • Year and Date: 20140300
• [Presentation] Rapid and high sensitive detection system for malaria parasite using a cell microarray chip (2013)
  • Author(s): Yatsushiro S, Yamamura S, Kataoka M
  • Organizer: PITTCON 2013
  • Place of Presentation: フィラデルフィア(米国)
  • Year and Date: 20130300
• [Presentation] 細胞チップを用いたマラリア迅速検出法の確立 (2011)
  • Author(s): 八代聖基
  • Organizer: 第33回薬剤と生体膜の相互作用シンポジウム
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 20111000
• [Remarks]
  • URL: http://unit.aist.go.jp/hri/