Analysis of AICD in neurogenesis

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kagoshima University
• Principal Investigator: TAKEDA YASUO 鹿児島大学, 医学部・歯学部附属病院, 教授 (60245462)
• Co-Investigator(Kenkyū-buntansha): IKEDA Ryuji 鹿児島大学, 医学部・歯学部附属病院, 准教授 (50398278)
• Research Collaborator: XIAO Zhi-cheng Monash大学, 医学部, 教授 ; FUTAGAWA Toshitaka 鹿児島大学, 医学部・歯学部附属病院, 薬剤師 ; TAKAHAMA Kazuhiro 鹿児島大学, 医学部・歯学部附属病院, 薬剤師 ; 益田 将吾 鹿児島大学, 医学部・歯学部附属病院, 薬剤師
• Project Period (FY): 2011 – 2013
• Keywords: アミロイド前駆蛋白質 / アルツハイマー病 / 神経分化 / AICD

Research Abstract

Alzheimer's disease(AD)is a neurodegenerative disease and the most common form of dementia. Amyloid precursor protein(APP)plays a central role in the pathogenesis of AD. APP is type I transmembrane protein with a large extracellular domain and a short cytoplasmic domain(AICD59, AICD). AICD negatively modulates neurogenesis in neural precursor cells. However, the molecular mechanism of AICD have not been identified yet. In this study, we investigated the role of AICD in neuronal cell differentiation of P19 embryonic carcinoma cells. We have shown that P19 cells with induction Retinoic acid(RA) and transient overexpression of AICD could inhibit neural differentiation using qunantitative RT-PCR. Oct4, pluripotency stem cell marker was upregulated during the RA aggregated stage of AICD overexpressing P19 cells.Our data suggest that AICD may be involved in inhibited effect of neural differentiation during developmental brain.

Research Products

• [Journal Article] Factors involved in the cisplatin resistance of KCP‑4 human epidermoid carcinoma cells (2014)
  • Author(s): Oiso S, Takayama Y, Nakazaki R, Matsunaga N, Motooka C, Yamamura A, Ikeda R, Nakamura K, Takeda Y, Kariyazono H
  • Journal Title: Oncol Rep Volume: 31(2) Pages: 719-726
  • Peer Reviewed
• [Journal Article] Regulation of major vault protein expression by upstream stimulating factor 1 in SW620 human colon cancer cells (2014)
  • Author(s): Ikeda R, Nishizawa Y, Tajitsu Y, Minami K, Mataki H, Masuda S, Furukawa T, Akiyama S, Yamada K, Takeda Y
  • Journal Title: Oncol Rep Volume: 31(1) Pages: 197-201
  • Peer Reviewed
• [Journal Article] Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells (2013)
  • Author(s): Tajitsu Y, Ikeda R, Nishizawa Y, Mataki H, Che XF, Sumizawa T, Nitta M, Yamaguchi T, Yamamoto M, Tabata S, Akiyama S, Yamada K, Furukawa T, Takeda Y
  • Journal Title: Int J Mol Med Volume: 32(3) Pages: 703-708
  • Peer Reviewed
• [Journal Article] Molecular basis for the regulation of hypoxia-inducible factor-1αlevels by 2-deoxy-D-ribose (2013)
  • Author(s): Ikeda R, Tabata S, Tajitsu Y, Nishizawa Y, Minami K, Furukawa T, Yamamoto M, Shinsato Y, Akiyama S, Yamada K, Takeda Y
  • Journal Title: Oncol Rep Volume: 30(3) Pages: 1444-1448
  • Peer Reviewed
• [Journal Article] Reduction in the incidence of MRSA with use of alcohol-based hand rub solutions and gloves (2012)
  • Author(s): Kazuaki Matsumoto, Akari Shigemi, Keiko Yaji, Yoshihiro Shimodozono, Yasuo Takeda, Kazuro Ikawa, Norifumi Morikawa, Hiroaki Miyanohara, Hideki Kawamura, Michiyo Orita, Koichi Tokuda, Junichiro Nishi, Katsushi Yamada
  • Journal Title: Journal of Infection and Chemotherapy Volume: 18 Pages: 269-271
  • Peer Reviewed
• [Journal Article] Pharmacokinetics of ampicillin- sulbactam and the renal function-based optimization of dosing regimens for prophylaxis in patients undergoing cardiovascular surgery (2012)
  • Author(s): Yuta Yokoyama, Kazuaki Matsumoto, Hiroyuki Yamamoto, Yoshifumi Iguro, Yutaka Imoto, Kazuro Ikawa, Norifumi Morikawa, Shiro Ishida, Yoshiro Okano, Erika Watanabe, Yoshihiro Shimodozono, Katsushi Yamada, Yasuo Takeda
  • Journal Title: Journal of Infection and Chemotherapy Volume: 18 Pages: 878-882
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Sho Tabata, Ryuji Ikeda, Masatatu Yamamoto, Tatsuhiko Furukawa, Takuya Kuramoto, Yasuo Takeda, Katsushi Yamada, Misako Haraguchi, Yasuhiko Nishioka, Saburo Sone, Shin-Ichi Akiyama
  • Journal Title: Oncol Rep Volume: 28 Pages: 895-902
  • Peer Reviewed
• [Journal Article] p53R2 is a prognostic factor of melanoma and regulates proliferation and chemosensitivity of melanoma cells (2012)
  • Author(s): Shigeto Matsushita, Ryuji Ikeda, Tomoko Fukushige, Yusuke Tajitsu, Kanayo Gunshina, Hiroshi Okumura, Mina Ushiyama, Shin-ichi Akiyama, Kazuhiro Kawai, Yasuo Takeda, Katsushi Yamada, Takuro Kanekura
  • Journal Title: Journal of Dermatological Science Volume: 68 Pages: 19–24
  • Peer Reviewed
• [Journal Article] An initial dosing method for teicoplanin based on the area under the serum concentration-time curve (AUC) required for MRSA eradication (2011)
  • Author(s): Naoko Kanazawa, Kazuaki Matsumoto, Kazuro Ikawa, Tomohide Fukamizu, Akari Shigemi, Keiko Yaji, Yoshihiro Shimodozono, Norifumi Morikawa, Yasuo Takeda, Katsushi Yamada
  • Journal Title: Journal of Infection and Chemotherapy Volume: 17 Pages: 297-300
  • Peer Reviewed
• [Journal Article] Effect of 5-Fluorouracil Treatment on SN-38 Absorption from Intestine in Rats (2011)
  • Author(s): Yoshihiko Shibayama, Yoshitaka Iwashita, Yoshimi Yoshikawa, Tomoko Kondo, Ryuji Ikeda, Yasuo Takeda, Takayuki Osada, Mitsuru Sugawara, Katsushi Yamada, Ken Iseki
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 34 Pages: 1418-1425
  • Peer Reviewed
• [Presentation] 神経接着関連分子Caspr1はMMP-9の発現低下を介してグリオーマ細胞の浸潤を抑制する (2013)
  • Author(s): 益田将吾,二川俊隆,山口沙織,山田勝士,池田龍二,武田泰生
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜市
  • Year and Date: 20130327-30
• [Presentation] 高浸透圧によって誘導されるMajor vault protein (MVP)の発現亢進機序 (2013)
  • Author(s): 田實裕介,池田龍二,西澤由紀彦,山口辰哉,山本雅達,田畑祥,秋山伸一,山田勝士,古川龍彦,武田泰生
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 20130321-23
• [Presentation] A Caspr4/LNX2 signaling pathway promotes neurogenesis (2013)
  • Author(s): Toshitaka Futagawa, Masuda Shogo, Katsushi Yamada, Ryuji Ikeda, Yasuo Takeda
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 20130321-23
• [Presentation] 神経接着関連分子Caspr1はグリオブラストーマ細胞の浸潤を調節する (2013)
  • Author(s): 益田将吾,二川俊隆,山口沙織,山田勝士,池田龍二,武田泰生
  • Organizer: 第26回鹿児島ブレインサイエンスカンファランス
  • Place of Presentation: 鹿児島市
  • Year and Date: 2013-10-22
• [Presentation] Molecular basis for the induction of vaults by anti-cancer agents, and the role of vaults in resistance to anti-cancer sagents (2012)
  • Author(s): Ryuji Ikeda, Yusuke Tajitsu, Yukihiko Nishizawa, Nariaki Tomiyama, Hironori Mataki, Katsushi Yamada, Yasuo Takeda
  • Organizer: 第6回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 京都市
  • Year and Date: 20121123-24
• [Presentation] グリオーマ細胞の浸潤・転移におけるContactin associated protein(Caspr)1の役割 (2012)
  • Author(s): 益田将吾,二川俊隆,山口沙織,池田龍二,山田勝士,武田泰生
  • Organizer: 医療薬学フォーラム2012/第20回クリニカルファーマシーシンポジウム
  • Place of Presentation: 福岡市
  • Year and Date: 20120714-15
• [Presentation] グリオーマ細胞の移動・浸潤におけるCaspr1の役割 (2012)
  • Author(s): 益田将吾,二川俊隆,山口沙織,山田勝士,池田龍二,武田泰生
  • Organizer: 第65回日本薬理学会西南部会
  • Place of Presentation: 熊本市
  • Year and Date: 2012-11-23
• [Presentation] 神経接着関連分子Caspr4の脳形成過程における役割 (2012)
  • Author(s): 二川俊隆,益田将吾,鬼丸貴裕,山田勝士,池田龍二,武田泰生
  • Organizer: 第65回日本薬理学会西南部会
  • Place of Presentation: 熊本市
  • Year and Date: 2012-11-23
• [Presentation] Hyperosmotic stress up-regulates the expression of major vault protein in SW620 human colon cancer cells (2011)
  • Author(s): Ryuji Ikeda, Yusuke Tajitsu, Yukihiko Nishizawa, Sho Tabata, Mina Ushiyama, Tatsuhiko Furukawa, Shin-ichi Akiyama, Katsushi Yamada, Yasuo Takeda
  • Organizer: 第5回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 名古屋市
  • Year and Date: 20111126-27
• [Presentation] 抗がん剤耐性機序の解明に向けて (2011)
  • Author(s): 池田龍二,武田泰生,山田勝士
  • Organizer: 第5回日本緩和医療薬学会年会
  • Place of Presentation: 千葉市
  • Year and Date: 20110924-25
• [Book] 月刊薬事2012年8月号(Vol. 54 No.8),特集薬剤耐性Up-to-Date -感染症・がん領域を中心に-抗がん剤耐性機序の解明に向けて (2012)
  • Author(s): 池田龍二,武田泰生,他
• [Book] 医薬ジャーナル2012年12月号(Vol. 48 No. 12),特集生活習慣病としての肝疾患～非アルコール性脂肪性肝疾患(NAFLD)/非アルコール性脂肪肝炎(NASH)の現状と対応～11.新規の生活習慣病治療薬～インクレチン関連薬の作用機序とNAFLD/NASH治療への応用～ (2012)
  • Author(s): 近藤智子,武田泰生, 他