2013 Fiscal Year Final Research Report
Identification of the novel genes associated with vascular morphogenesis
| Project/Area Number |
23590222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Iwate Medical University |
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Principal Investigator |
KIMURA Eiji 岩手医科大学, 医学部, 助教 (50405750)
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| Co-Investigator(Kenkyū-buntansha) |
ISOGAI Sumio 岩手医科大学, 医学部, 准教授 (60212966)
HITOMI Jiro 岩手医科大学, 医学部, 教授 (00218728)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 血管形成 / ゼブラフィッシュ / マイクロアレイ / etsrp/etv2 |
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| Research Abstract |
Vascular formation progressed with a regular time course to form a uniform structure. However the associated genes of vascular morphogenesis are still poorly uncovered. To address this issue, we performed microarray analysis of etsrp/etv2 deficient zebrafish embryos. As a result, we identified 165 genes that were up-regulated greater than 1.5 fold by silencing etsrp/etv2 expression. Furthermore, we analyzed their expression patterns to evaluate whether these genes were associated with the cranial and/or truncal vascular formation by in silico analysis using ZFIN database, and selected 11 genes which were expressed in the specific region associated with the vascular formation. We also performed in situ hybridization for 68 selected genes which precise expression patterns were not obtained, and succeeded to demonstrate three new expression patterns. We are now planning to knock out these genes using CRISPR/Cas9 system to analyze their function for the vascular formation.
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[Presentation] 19th Japanese Medaka and Zebrafish MeetingApplication of IR-LEGO (Infrared Laser-Evoked Gene Operator) to the model animals and plants as Collaborative research projects in NIBB2013
Author(s)
Atsuko Shimada, Takuya Kaneko, Teruhiro Okuyama, Yasuko Isoe, Eiji Kimura, Aiko Kawasumi, Chikashi Nagayama, Misako Saida-Taniguchi, Atsushi Shimizu, Hitoshi Yokoyama, Yoshihiro Morishita, Toshinori Hayashi, Hiroko Urawa, Koji Tamura, Masato Kinoshita, Kiyoshi Naruse, Hiroyuki Takeda, Hideaki Takeuchi, Yasuhiro Kamei
Organizer
19th Japanese Medaka and Zebrafish Meeting
Place of Presentation
Sendai, Poster
Year and Date
20130920-21
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[Presentation] Application of IR-LEGO (Infrared Laser-Evoked Gene Operator) to the Model Animals and Plants as Collaborative Research Projects in NIBB2012
Author(s)
Yasuhiro Kamei, Hiroko Urawa, Kazuhiko Yamamoto, Takuya Kaneko, Eiji Kimura, Misako Saida-Taniguchi, Tomomi Suzuki, Akira Nagatani, Atsuko Shimada, Hiroyuki Takeda
Organizer
The 35th Annual Meeting of the Molecular Biology Society of Japan
Place of Presentation
Fukuoka, Poster
Year and Date
20121211-14
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[Presentation] IR-laser-mediated gene induction system2012
Author(s)
Misako Saida-Taniguchi, Takuya Kaneko, Atsuko Shimada, Hiroyuki Takeda, Eiji Kimura, Hiroko Urawa and Yasuhiro Kamei
Organizer
18th Japanese Medaka and Zebrafish Meeting
Place of Presentation
Kyoto, Poster
Year and Date
20120922-23
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[Presentation] 赤外レーザーによる遺伝子発現システム(IR-LEGO)の様々なモデル生物への応用2011
Author(s)
亀井保博,浦和博子,山本和彦,兼子拓也,木村英二,出口友則,弓場俊輔,北野健,尾田正二,三谷啓志,斎田-谷口美佐子,鈴木友美,長谷あきら,島田敦子,武田洋幸,岡田清孝
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜, ポスター
Year and Date
20111213-16
