2013 Fiscal Year Final Research Report
Study on a regulatory mechanism of the maturation of dendritic spines and the synaptic plasticity by lipid-related molecules
| Project/Area Number |
23590232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Fukui |
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Principal Investigator |
XIE Min-Jue 福井大学, 医学部, 助教 (40444210)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Makoto 大阪大学, 連合小児発達学研究科, 教授 (10222019)
KURODA Kazuki 福井大学, 医学部, 助教 (60557966)
IGUCHI Tokuichi 大阪大学, 医学系研究科, 助教 (60509305)
KOMADA Munekazu 愛知学院大学, 歯学部, 助教 (90523994)
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| Project Period (FY) |
2011 – 2013
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| Keywords | シナプス形成 / シナプス可塑性 / LL5β / PSD / LTD |
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| Research Abstract |
Synapse function and plasticity depend on the morphology of dendritic spine. Here, we report that Phldb2 (pleckstrin homology-like domain, family B, member 2, LL5beta), one binding partner for a well-known actin-cross-linking protein Filamin A, works as a positive regulator of spine maturation. We generated LL5beta knockout mice and found that a proportion of immature spines (filopodia and thin spines) increased in the hippocampus in vivo, which is consistent with our previous observations with LL5beta knocked-down cultured hippocampal neurons. Next, we asked whether or not LL5beta is involved in synaptic plasticity. We observed that NMDA-induced AMPA receptor endocytosis and low-frequency stimulation-induced long-term depression were blocked in hippocampal neurons of the LL5beta knockout mice. Therefore, it is likely that LL5beta plays an important role for the synaptic plasticity and the maturation of dendritic spines.
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