2013 Fiscal Year Final Research Report
Ion channel coupling and membrane plasticity in nucleus and mitochondria
| Project/Area Number |
23590254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Tohoku University |
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Principal Investigator |
MARUYAMA Yoshio 東北大学, 医学(系)研究科(研究院), 教授 (00133942)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Yoshimichi 東北大学, 大学院医学系研究科, 講師 (60455780)
KAZAMA Itsurou 東北大学, 大学院医学系研究科, 助教 (60593978)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 核膜・小胞体膜 / イオンチャネル / 膜容量 / 膜可塑性 / 膵外分泌腺 |
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| Research Abstract |
Fixing the lumen Ca2+-concentration at over 10 micro molar concentration in a nuclear/endoplasmic reticulum preparation (nuclear envelope: NE), which is subjected into whole-NE configuration of the patch-clamp recording techniques, I observed increases of membrane electrical capacitance, the measure of the membrane area. The membrane capacitance, recorded under in situ patch-clamp condition, fluctuated microscopically. The result suggests that the NE membrane system shows a characteristic undulation reflecting temporal membrane vesiculation and collapse, and the process is under control of the lumen Ca2+ concentration. The NE membrane is rich in ion- and water- channels. Those active membrane may component contribute to maintain NE assembly, the structure of the lumen and the nuclear membranes. Further, I observed with laser confocal microscopy in the NE preparation, a thick membrane accumulation, stacking into layers at a small spot, assumingly corresponding to the nucleic pore.
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[Journal Article] Decreased Expression of a Novel Prostaglandin Transporter, OAT-PG, Facilitates Renocortical PGE(2) Accumulation during Rat Pregnancy2013
Author(s)
Kazama I, Matsubara M, Kanai Y, Hatano R, Asano S, Endo Y, Toyama H, Ejima Y, Kurosawa S, Maruyama Y
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DOI
Peer Reviewed
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