2013 Fiscal Year Final Research Report
High time resolution FRET analysis of the activation of G protein
| Project/Area Number |
23590270
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KUBO Yoshihiro 生理学研究所, 分子生理研究系, 教授 (80211887)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Gタンパク質共役型受容体 / FRET / Gタンパク質 |
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| Research Abstract |
G protein coupled receptors (GPCRs) are the important signaling molecules which are activated upon the binding with the specific ligand and trigger the downstream signaling through activating G protein. These initial processes of GPCR signaling, the activation of receptor and the following G protein binding, were analyzed by using a fluorescence resonance energy transfer (FRET) technique. With the high time resolution FRET analysis, we found that binding of G protein stabilizes the active conformations of GPCRs and slows the deactivation of the activated receptor. Further FRET studies revealed that the effects of G protein binding differ depending on the type of the coupled receptors.
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