2013 Fiscal Year Final Research Report
Role of neuronal PAS domain 4 (Npas4) in psychiatric disorder.
| Project/Area Number |
23590299
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Nagoya University |
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Principal Investigator |
HIBI YOKO 名古屋大学, 医学部附属病院, 特任助教 (70295616)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Kiyofumi 名古屋大学, 医学部附属病院, 教授 (30303639)
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| Co-Investigator(Renkei-kenkyūsha) |
NAGAI Taku 名古屋大学, 医学部附属病院, 准教授 (10377426)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ストレス / Npas4 / 転写制御 / グルココルチコイド / プロモーター |
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| Research Abstract |
Neuronal PAS domain 4 (NPAS4) has recently been shown to regulate the development of GABAergic inhibitory synapses. We previously reported that Npas4 mRNA expression levels were reduced in the hippocampus of mice exposed to social isolation or restraint stress. In the present study, to investigate the transcriptional regulation of Npas4 by stress, we focused on the effect of glucocorticoids upon Npas4 transcription. Corticosterone treatment reduced Npas4 expression in the frontal cortex and hippocampus. Putative negative glucocorticoid response elements (GREs) were found -2000 to -1000 upstream of the Npas4 transcription initiation site. Npas4 promoter activity was increased by mutation of the negative GRE sequences. Restraint stress increased the binding of GR to Npas4 promoter region in the hippocampus. These results suggest that transcription of Npas4 is down-regulated by stress via the binding of agonist-bound GR to its promoter.
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Research Products
(30 results)
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[Journal Article] Deletion of SHATI/NAT8L increases dopamine D1 receptor on the cell surface in the nucleus accumbens, accelerating methamphetamine dependence2014
Author(s)
Toriumi K., Kondo M., Nagai T., Furukawa-Hibi Y., Mamiya T., Fukushima T., Takeda M., Nitta A., Yamada K., Nabeshima T.(他7名)
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Journal Title
Int. J . Neuropsychopharmacol
Volume: 17
Pages: 443-452
Peer Reviewed
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[Journal Article] Neuronal PAS domain protein 4 (Npas4) regulates neurite outgrowth and phosphorylation of synapsin I2013
Author(s)
Yun, J., Nagai, T., Furukawa-Hibi, Y., Kuroda, K., Kaibuchi, K. and Yamada, K.
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Journal Title
J . Biol. Chem
Volume: 288
Pages: 2655-2664
Peer Reviewed
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[Journal Article] Somatic mosaicism for oncogenic NRAS mutations in juvenile myelomonocytic leukemia2012
Author(s)
Doisaki, S., Muramatsu, H., Shimada, A., Furukawa-Hibi, Y., Yamada, K., Tanaka, M., Hama, A., Koike, K. and Kojima, S.(他16名)
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Journal Title
Blood
Volume: 120(7)
Pages: 1485-1488
Peer Reviewed
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[Journal Article] Using peripheral blood circulating DNAs to detect CpG global methylation status and genetic mutations in patients with myelodysplastic syndrome2012
Author(s)
Iriyama, C., Tomita, A., Furukawa-Hibi, Y., Yamada, K., Kiyoi, H., Naoe, T.(他2名)
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Journal Title
Biochem Biophys Res Commun
Volume: 419(4)
Pages: 662-669
Peer Reviewed
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[Journal Article] Periocular injection of in situ hydrogels containing Leu-Ile, an inducer for neurotrophic factors, promotes retinal ganglion cell survival after optic nerve injury2011
Author(s)
Nakatani, M., Shinohara, Y., Furukawa-Hibi, Y., Miyamoto, Y., Nitta, A.(他4名)
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Journal Title
Experimental Eye Research
Volume: 96(6)
Pages: 873-879
Peer Reviewed
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