2013 Fiscal Year Final Research Report

The significance of endothelin-1 and its receptors on nitric oxide production

Research Project

Project/Area Number	23590314
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	General pharmacology
Research Institution	Osaka University of Pharmaceutical Sciences
Principal Investigator	OHKITA MAMORU 大阪薬科大学, 薬学部, 准教授 (60449824)
Co-Investigator(Kenkyū-buntansha)	MATSUMURA Yasuo 大阪薬科大学, 薬学部, 教授 (40140230)
Project Period (FY)	2011 – 2013
Keywords	エンドセリン / 一酸化窒素
Research Abstract	There was no difference in NO production under basal condition between homozygous ETB deficient and wild-type rats. However, NOx output in the renal tissue by continuous infusion of L-arginine was significantly lower in homozygous than wild-type rats. This reduced converting activity of L-arginine to NO in homozygous rats was completely recovered by the acute administration of a selective ETA receptor antagonist. These results suggest that the augmentation of ET-1/ETA receptor-mediated actions involves in the decline of renal NO production under ETB receptor dysfunction. On the other hand, serum NOx levels in L-arginine treated homozygous rats were significantly increased compared to that of wild type rats, and that the expression of relative factors in NO production also significantly increased in the aortic tissue, suggesting that different mechanisms would control NO production in kidney and vasculature of homozygous rats, respectively.