Electrolytes gradient dependent nutritional absorption.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: TAMURA Atsushi 大阪大学, 生命機能研究科, 准教授 (00362525)
• Project Period (FY): 2011 – 2013
• Keywords: タイトジャンクション / クローディン / イオンホメオスターシス

Research Abstract

Multicellular organism is surrounded by the epithelial cell sheets. Epithelial cell sheet is composed of epithelial cells which are adhered each other by cell-cell adhesion complex. Epithelial cell sheets cover inside and outside of our body and the play a biological specific role as organ like skin, intestine and so on. In this research, I analyzed the tight junction mechanism, by which biologically adequate microenvironment can be formed in our body. In this study, I focused especially on small intestine using clausdin-2 and -15 knockout mice. The claudin-2 and -15 knockout mice showed imbalanced electrolytes of intestine induced malnutrition and infantile death.

Research Products

• [Journal Article] The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK (2013)
  • Author(s): Yano T, Matsui T, Tamura A, Uji M, Tsukita S
  • Journal Title: J Cell Biol Volume: 203 Pages: 605-614
  • DOI: 10.1083/jcb.201304194
• [Journal Article] Loss of claudins 2 and 15 from mice causes defects in paracellular Na+ flow and nutrient transport in gut and leads to death from malnutrition (2013)
  • Author(s): Wada M, Tamura A, Takahashi N, Tsukita S
  • Journal Title: Gastroenterology Volume: 144 Pages: 369-380
  • DOI: 10.1053/j.gastro.2012.10.035
• [Journal Article] Ezrin is required for the functional regulation of the epithelial sodium proton exchanger, NHE3 (2013)
  • Author(s): Hayashi H, Tamura A, Krishnan D, Tsukita S, Suzuki Y, Kocinsky HS, Aronson PS, Orlowski J, Grinstein S, Alexander RT
  • Journal Title: PLoS One Volume: 8 Pages: e55623
  • DOI: 10.1371/journal.pone.0055623
• [Journal Article] EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins (2012)
  • Author(s): Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, Yashiro K, Tsukita S, Hamada H
  • Journal Title: Dev Biol Volume: 371 Pages: 136-145
  • DOI: 10.1016/j.ydbio.2012.07.005
• [Journal Article] Claudin-based paracellular proton barrier in the stomach (2012)
  • Author(s): Tamura A, Yamazaki Y, Hayashi D, Suzuki K, Sentani K, Yasui W, Tsukita S
  • Journal Title: Ann N Y Acad Sci Volume: 1258 Pages: 108-114
  • DOI: 10.1111/j.1749-6632.2012.06570.x
• [Journal Article] Deficiency of claudin-18 causes paracellular H+ leakage, up-regulation of interleukin-1β, and atrophic gastritis in mice (2012)
  • Author(s): Hayashi D, Tamura A, Tanaka H, Yamazaki Y, Watanabe S, Suzuki K, Suzuki K, Sentani K, Yasui W, Rakugi H, Isaka Y, Tsukita S
  • Journal Title: Gastroenterology Volume: 142 Pages: 292-304
  • DOI: 10.1053/j.gastro.2011.10.040
• [Journal Article] Coordinated ciliary beating requires Odf2-mediated polarization of basal bodies via basal feet (2012)
  • Author(s): Kunimoto K, Yamazaki Y, Nishida T, Shinohara K, Ishikawa H, Hasegawa T, Okanoue T, Hamada H, Noda T, Tamura A, Tsukita S, Tsukita S
  • Journal Title: Cell Volume: 148 Pages: 189-200
  • DOI: 10.1016/j.cell.2011.10.052
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
  • Journal Title: Cell Tissue Res Volume: 343 Pages: 637-648
  • DOI: 10.1007/s00441-010-1117-y
• [Presentation] 腸管特異的Claudin-7欠損はタイトジャンクション透過性を変化させ, 大腸炎を惹起する (2013)
  • Author(s): 田中啓雄, 武知真希, 田村淳, 山崎裕自, 月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-12-14
• [Presentation] 肝・胆道系の胆汁フロー調節におけるクローディンの役割 (2013)
  • Author(s): 今里光伸, 山崎裕自, 田中啓雄, 松本健吾, 国本晃司, 田村淳, 渡辺光博, 月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-12-14
• [Presentation] Role of tight junction in biological systems-more than a simple paracellular barrier in inflammation and metabolism (2013)
  • Author(s): Atsushi Tamura, and Sachiko Tsukita
  • Organizer: IUPS
  • Place of Presentation: Birmingham. England
  • Year and Date: 2013-07-26
• [Presentation] Biological function of paracellular barrier - forming claudins and claudin-deficiency-related inflammation (2013)
  • Author(s): 田村淳, 月田早智子
  • Organizer: 日本病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2013-06-06
• [Presentation] Claudin-18 is according to structure tight junction not to leakage of gastric acid in mouse stomach (2013)
  • Author(s): Koya Suzuki, Atsushi Tamura, Daisuke Hayashi, Yuji Ymamazaki,and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structual Physiology
  • Place of Presentation: 名古屋
  • Year and Date: 2013-01-22
• [Presentation] Intestine specific loss of Claudin-7 causes tight junction permeability change and intestinal inflammation (2013)
  • Author(s): Hiroo Tanaka, Maki Takechi, Atsushi Tamura,Yuji Yamazaki, and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structual Physiology
  • Place of Presentation: 名古屋
  • Year and Date: 2013-01-22
• [Presentation] Loss of Claudin-2 and 15 from Mice Causies Defects in Paracellular Na+ Flow and Nutritional Transport in Lumen and Leads to Death from Malnutrition (2013)
  • Author(s): Masami Wada, Atsushi Tamura,Nobuyuki Takahashi, and Sachiko Tsukita
  • Organizer: Nagoya Symposium Frontiers in Structual Physiology
  • Place of Presentation: 名古屋
  • Year and Date: 2013-01-22
• [Presentation] 上皮細胞間接着を起としたアピカル細胞骨格構築 (2013)
  • Author(s): 月田早智子, 矢野智樹, 立石和博, 田村淳, 山崎裕自
  • Organizer: 2013生体運動研究合同班会議
  • Place of Presentation: 広島
  • Year and Date: 2013-01-12
• [Presentation] 上皮細胞間タイトジャンクションを起点と下上皮細胞シートの高次構築 (2012)
  • Author(s): 矢野智樹, 立石和博, 伊藤雅彦, 小倉祐輝, 田村淳, 山崎裕自, 月田早智子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] The role of claudin in regulating the liver-biliary system International Conference Merida 2012 (2012)
  • Author(s): Yuji Yamazaki, Mitsunobu Imasato,Hiroo Tanaka, Kengo Matsumoto, Atsushi Tamura, Koshi Kunimoto,Mitsuhiro Watanabe, Sachiko Tsukita
  • Organizer: Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
  • Place of Presentation: Mexico
  • Year and Date: 2012-11-14
• [Presentation] Coodinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet (2012)
  • Author(s): Koshi Kunimoto, Yuji Yamazaki, Tomoki Nishida, Kyosuke Shinohara, Hiroaki Ishikawa, Toshiaki Hasegawa, Takeshi Okanouem Hiroshi Hamada, Tetsuo Noda ,Atsushi Tamura, Shoichiro Tsukita Sachiko Tsukita
  • Organizer: 大阪バイオサイエンス研究所マンスリーレクチャー
  • Place of Presentation: 大阪
  • Year and Date: 2012-04-11
• [Presentation]
  • Author(s): Tomoki Yano, Masahiko Ito, Takeshi Matsui, Atsushi Tamura, Katsushi Owaribe, Masami Uji, Yuji Yamazaki, Sachiko Tsukita
  • Organizer: ASCB 2012 annual meeting
  • Place of Presentation: Sanfrancisco
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/tsukita/