2013 Fiscal Year Final Research Report
Epigenetic transcriptional regulation by the nuclear factor Ikappab-zeta
| Project/Area Number |
23590338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
YAMAZAKI Soh 九州大学, 医学(系)研究科(研究院), 講師 (70315084)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 転写 / エピジェネティクス |
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| Research Abstract |
The transcription factor NF-kappaB is essential for expression of proinflammatory cytokines and antimicrobial proteins upon microbial infection. IkappaB-zeta is a nuclear protein required for expression of a subset of NF-kappaB-target genes. In the present study, I attempted to elucidate transcriptional regulation by IIkappaB-zeta in the chromatin context. I found that expression of two genes, both encoding antimicrobial proteins, was synergistically induced by lipopolysaccharide and glucocorticoid. I specified cis-elements responsible for the transcriptional activation by the two stimulants, and revealed that upon stimulation NF-kappaB, glucocorticoid receptor, and chromatin remodeling factors cooperatively associate with the cis-elements in an Ikappab-zeta-dependent manner.
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