Analysis in the regulation mechanism of oxidative stress by sphingolipid

2014 Fiscal Year Final Research Report

• Project/Area Number: 23590340
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kumamoto Health Science University (2013-2014); Kumamoto University (2011-2012)
• Principal Investigator: YANO Masato 熊本保健科学大学, 保健科学部, 准教授 (60315299)
• Co-Investigator(Renkei-kenkyūsha): OIKE Yuichi 熊本大学, 大学院医学薬学研究部, 教授 (90312321)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: スフィンゴミエリン合成酵素 / ミトコンドリア / 酸化ストレス / ミトコンドリアストレス応答 / ABCB10 / TMEM65

Outline of Final Research Achievements

Although it has been suggested that disorder of sphingolipid metabolism is involved in the pathogenesis of a variety of disease, the mechanism has not been fully elucidated. In this study, we analyzed sphingomyelin synthase 1 (SMS1, a sphingolipid metabolizing enzyme) deficient mice, and found that lipodystrophy observed in this mice was probably caused not only by increased oxidative stress, but also by reduction of mitochondrial function, reduced activity of lipoprotein lipase, and a decrease in fatty acid uptake. Moreover, we found that the mitochondrial stress response pathway was activated in the adipocytes of SMS1-deficient mice, and performed functional analysis of the candidate factors involved in this pathway.

Free Research Field

生化学