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2013 Fiscal Year Final Research Report

Function of novel BRCA2-associating proteins in centrosomal regulation and DNA repair

Research Project

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Project/Area Number 23590355
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathological medical chemistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TAKENAKA Katsuya  東京医科歯科大学, 難治疾患研究所, 助教 (20378706)

Co-Investigator(Kenkyū-buntansha) OGI Tomoo  長崎大学, 7原爆後障害医療研究所, 准教授 (80508317)
Project Period (FY) 2011 – 2013
Keywords乳癌 / BRCA / 中心体 / 画像認識
Research Abstract

BRCA2 germline mutations account for the majority of heredity breast and ovarian cancer. A major function of BRCA2 is known as a regulator of homologous recombination in DNA damage repair. In addition, BRCA2 also plays an important role in centrosomal regulation, whose dysfunction might be involved in
the tumorigenesis of breast cancer. Even so, detail molecular mechanism is
not yet uncovered. In the present study, we tried to locate a molecular region of BRCA2 responsible for this function. Series of BRCA2 fragments were exogenously expressed in human cultured cells. Numbers of centrosome in a cell are counted to see if an overexpression of a specific fragment could impair a numerical integrity of centrosomes. For this purpose we developed an automated centrosome-counting system based on image recognition, which allows us to judge a large number of transfected cells without human bias.

  • Research Products

    (4 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] BRCA2 phosphorylated by PLK1 moves to the midbody to regulate cytokinesis mediated by nonmuscle myosin IIC2014

    • Author(s)
      Miho Takaoka, Hiroko Saito, Katsuya Takenaka, Yoshio Miki, Akira Nakanishi
    • Journal Title

      Cancer Research

      Volume: 74 Pages: 1518-1528

    • DOI

      10.1158/0008-5472.CAN-13-0504

    • Peer Reviewed
  • [Journal Article] Mutations in UVSSA cause UV-sensitive syndrome and impair RNA polymerase IIo processing in transcription-coupled nucleotide-excision repair2012

    • Author(s)
      Yuka Nakazawa, Kensaku Sasaki, Norisato Mitsutake, Michiko Matsuse, Mayuko Shimada, Tiziana Nardo, Yoshito Takahashi, Kaname Ohyama, Kosei Ito, Hiroyuki Mishima, Masayo Nomura, Akira Kinoshita, Shinji Ono, Katsuya Takenaka, Ritsuko Masuyama, Takashi Kudo, Hanoch Slor, Atsushi Utani, Satoshi Tateishi, Shunichi Yamashita, Miria Stefanini, Alan R. Lehmann, Koh-ichiro Yoshiura, Tomoo Ogi
    • Journal Title

      Nature Genetics

      Volume: 44 Pages: 586-592

    • DOI

      10.1038/ng.2229

    • Peer Reviewed
  • [Presentation] 中心体複製制御における乳癌原因遺伝子BRCA2とその結合分子の役割2013

    • Author(s)
      手代木翔太, 三木義男, 竹中克也
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      20131003-05
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/mri

URL: 

Published: 2015-07-16  

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