2013 Fiscal Year Final Research Report
Spatio-temporal analysis of cell membrane molecules involved in the enhancement of malignant properties by glycosphingolipids
| Project/Area Number |
23590371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Chubu University |
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Principal Investigator |
FURUKAWA Keiko 中部大学, 生命健康科学部, 教授 (50260732)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Kenichi 京都大学, 物質−細胞統合システム拠点, 准教授 (50423059)
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| Project Period (FY) |
2011 – 2013
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| Keywords | スフィンゴ糖脂質 / メラノーマ / EMARS反応 / １分子観察 / HGF刺激 / 接着刺激 / convergence |
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| Research Abstract |
We analyzed effects of GD3 expression on the cell signals triggered by hepatocyte growth factor (HGF)/Met interaction and by adhesion to collagen type I. The simultaneous treatment with both of them resulted in markedly increased activation of Akt and Erk in GD3+ cells. These results suggested that GD3 plays a crucial role in the convergence of PI3K-Akt and Ras-Erk signals, leading to the synergistic effects of those signals on malignant properties of melanomas. To search molecules existing in the vicinity of GD3 in living cells, enzyme-mediated activation of radical sources (EMARS) combined with mass spectrometry was performed. Neogenin and Integrin were identified to be interacting molecules with GD3 on lipid rafts of the cell membrane. We also tried spatio-temporal analysis of cell membrane molecules by single molecule observation, showing that glycosphingolipid GM1 specifically forms homodimer on the cell surface. Single molecule observation of GD3 on the membrane is now on-going.
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Research Products
(35 results)
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[Journal Article] β4GalT6 is involved in the synthesis of lactosylceramide with less intensity than β4GalT52013
Author(s)
Tokuda N., Numata S., Li X, Nomura T., Takizawa M., Kondo Y., Yamashita Y., Hashimoto N., Kiyono T., Urano T., Furukawa K., Furukawa K.
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Journal Title
Glycobiology
Volume: 23
Pages: 1175-1183
DOI
Peer Reviewed
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[Journal Article] TLR4-MD-2 complex is negatively regulated by an endogenous ligand, globotetraosylceramide2013
Author(s)
Kondo Y., Ikeda K, Tokuda N., Nishitani C., Ohto U., Akashi-Takamura S., Ito Y., Uchikawa M., Kuroki Y., Taguchi R., Miyake K., Zhang Q., Furukawa K., Furukawa K.
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Journal Title
Proc Natl Acad Sci
Volume: 110
Pages: 4714-4719
DOI
Peer Reviewed
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[Journal Article] Functional activation of Src family kinase yes protein is essential for the enhanced malignant properties of human melanoma cells expressing ganglioside GD32011
Author(s)
Hamamura K., Tsuji M., Hotta H., Ohkawa Y., Takahashi M., Shibuya H., Nakashima H., Yamauchi Y., Hashimoto N., Hattori H., Ueda M., Furukawa K., Furukawa K.
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Journal Title
J Biol Chem
Volume: 286
Pages: 18526-18537
DOI
Peer Reviewed
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[Journal Article] Membrane sialidase NEU3 is highly expressed in human melanoma cells promoting cell growth with minimal changes in the composition of gangliosides2011
Author(s)
Miyata M., Kambe M., Tajima O., Moriya S., Sawaki H., Hotta H., Kondo Y., Narimatsu H., Miyagi T., Furukawa K., Furukawa K.
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Journal Title
Cancer Sci
Volume: 102
Pages: 2139-2149
DOI
Peer Reviewed
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