2013 Fiscal Year Final Research Report
The basic study of molecular targeted therapy via pancreatic duct tight junctions by using a novel culture system of human pancreatic duct epithelial cells
| Project/Area Number |
23590404
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MURATA Masaki 札幌医科大学, 医学部, 講師 (10404592)
TAKASAWA Akira 札幌医科大学, 医学部, 助教 (00593021)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ヒト正常膵管上皮細胞 / 膵癌 / 分子標的治療 / PKC / claudin |
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| Research Abstract |
In the present study, using normal human pancreatic duct epithelial cells and cancer cell lines. we investigated the basic study targeting tight junction molecules claudin-4, -18 and a signal molecule PKC that highly expressed in pancreatic cancer. Claudin-4 and -18 were regulated via a PKCalpha signal transduction pathway in both human pancreatic duct epithelial cells and cancer cells. The cytotoxic effects of CPE via claudin-4 are thought to be useful as a novel therapeutic tool for pancreatic cancer. The cytotoxicity was not observed at any concentration of CPE, whereas in pancreatic cancer cells, CPE had a dose-dependent cytotoxic effect. PKCalpha inhibitors represent potential therapeutic agents against human pancreatic cancer cells by the use of CPE cytotoxicity via claudin-4.
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[Journal Article]2013
Author(s)
Kojima T, Yamaguchi H, Ito T, Kyuno D, Kono T, Konno T, Sawada N
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Journal Title
Tissue Barriers
Volume: 1
Pages: e24894
DOI
Peer Reviewed
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[Journal Article] Protein kinase Cαinhibitor protects against downregulation of claudin-1 during epithelial-mesenchymal transition of pancreatic cancer2013
Author(s)
Kyuno D, Kojima T, Yamaguchi H, Ito T, Kimura Y, Imamura M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
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Journal Title
Carcinogenesis
Volume: 34
Pages: 1232-43
DOI
Peer Reviewed
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[Journal Article] Regulation of Tight Junctions in Upper Airway Epithelium2013
Author(s)
Kojima T, Go M, Takano K, Kurose M, Ohkuni T, Koizumi J, Kamekura R, Ogasawara N, Masaki T, Fuchimoto J, Obata K, Hirakawa S, Nomura K, Keira T, Miyata R, Fujii N, Tsutsumi H, Himi T, Sawada N
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Journal Title
BioMed Research International
Volume: 2013
Pages: 947072
DOI
Peer Reviewed
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[Journal Article] Curcumin prevents replication of respiratory syncytial virus and the epithelial responses to it in human nasal epithelial cells2013
Author(s)
Obata K, Kojima T, Masaki T, Okabayashi T, Yokota S, Hirakawa S, Nomura K, Takasawa A, Murata M, Tanaka S, Fuchimoto J, Fujii N, Tsutsumi H, Himi T, Sawada N
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Journal Title
PloS One
Volume: 8
Pages: e70225
DOI
Peer Reviewed
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[Journal Article] Marked induction of matrix metalloproteinase-10 by respiratory syncytial virus infection in human nasal epithelial cells2013
Author(s)
Hirakawa S, Kojima T, Obata K, Okabayashi T, Yokota SI, Nomura K, Obonai T, Fuchimoto J, Himi T, Tsutsumi H, Sawada N
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Journal Title
J Med Virol
Volume: 85
Pages: 2141-2150
DOI
Peer Reviewed
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[Journal Article] Humulone suppresses replication of respiratory syncytial virus and release of IL-8 and RANTES in normal human nasal epithelial cells2013
Author(s)
Fuchimoto J, Kojima T, Okabayashi T, Masaki T, Ogasawara N, Obata K, Nomura K, Hirakawa S, Kobayashi N, Shigyo T, Yokota SI, Fujii N, Tsutsumi H, Himi T, Sawada N
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Journal Title
Med Mol Morphol
Volume: 46
Pages: 203-209
DOI
Peer Reviewed
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[Journal Article] Hop water extract inhibits double-stranded RNA-induced thymic stromal lymphopoietin release from human nasal epithelial cells2012
Author(s)
Fuchimoto J, Kojima T, Kobayashi N, Ohkuni T, Ogasawara N, Masaki T, Obata K, Nomura K, Kondoh A, Shigyo T, Himi T, Sawada N
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Journal Title
Am J Rhinol Allergy
Volume: 26
Pages: 433-438
DOI
Peer Reviewed
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[Journal Article] An NF-κB signaling pathway via PKCδregulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells2011
Author(s)
Masaki T, Kojima T, Okabayashi T, Ogasawara N, Ohkuni T, Obata K, Takasawa A, Murata M, Tanaka S, Hirakawa S, Fuchimoto J, Ninomiya T, Fujii N, Tsutsumi H, Himi T, Sawada N
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Journal Title
Mol Biol Cell
Volume: 22
Pages: 2144-2156
DOI
Peer Reviewed
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[Journal Article] Transcriptional regulation of claudin-18 via specific protein kinase C signaling pathways and modification of DNA-methylation in human pancreatic cancer cells2011
Author(s)
Ito T, Kojima T, Yamaguchi H, Kyuno D, Kimura Y, Imamura M, Takasawa A, Murata M, Tanaka S, Hirata K, Sawada N
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Journal Title
J Cell Biochem
Volume: 112
Pages: 1761-1772
DOI
Peer Reviewed
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[Journal Article] Altered expression of claudin-1, claudin-7, and tricellulin regardless of human papilloma virus infection in human tonsillar squamous cell carcinoma2011
Author(s)
Kondoh A, Takano K, Kojima T, Ohkuni T, Kamekura R, Ogasawara N, Go M, Sawada N, Himi T
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Journal Title
Acta-Otolaryngol
Volume: 131
Pages: 861-868
DOI
Peer Reviewed
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[Journal Article] Poly(I : C) reduces expression of JAM-A and induces secretion of IL-8 and TNF-αvia distinct NF-κB pathways in human nasal epithelial cells2011
Author(s)
Ohkuni T, Kojima T, Ogasawara N, Masaki T, Fuchimoto J, Kamekura R, Koizumi JI, Ichimiya S, Murata M, Tanaka S, Himi T, Sawada N
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Journal Title
Toxicol Appl Pharmacol
Volume: 205
Pages: 29–38
DOI
Peer Reviewed
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[Journal Article] Epithelial barrier and antigen uptake in lymphoepithelium of human adenoids2011
Author(s)
Ogasawara N, Kojima T, Go M, Takano KI, Kamekura R, Ohkuni T, Koizumi JI, Masaki T, Fuchimoto J, Obata K, Kurose M, Shintani T, Sawada N, Himi T
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Journal Title
Acta Otolaryngol
Volume: 131
Pages: 116-123
DOI
Peer Reviewed
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