2013 Fiscal Year Final Research Report
Proteomics of Human Pancreatic Ductal Adenocarcinoma using FFPE Sections
| Project/Area Number |
23590408
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Osaka City University |
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Principal Investigator |
YUKO Kuwae 大阪市立大学, 医学(系)研究科(研究院), 病院講師 (50597557)
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| Co-Investigator(Kenkyū-buntansha) |
KAKEHASHI Anna 大阪市立大学, 大学院医学研究科, 講師 (60382222)
WAKASA Kennichi 大阪市立大学, 大学院医学研究科, 登録医 (60158574)
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| Co-Investigator(Renkei-kenkyūsha) |
WANIBUCHI Hideki 大阪市立大学, 大学院医学研究科, 教授 (90220970)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 浸潤性膵管癌 / バイオマーカー / プロテオーム |
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| Research Abstract |
The present study aimed to identify novel useful clinical biomarker at early stages and to elucidate the molecular background of carcinogenesis in human pancreatic ductal adenocarcinomas (PDACs). Proteomes of dissected PDACs and adjacent non-tumor pancreatic tissues from formalin-fixed and paraffin-embedded sections from 10 patients were analyzed using QSTAR Elite LC-MS/MS, ProteinPilot and Ingenuity Pathway Analysis (IPA). Expression of potential biomarker candidates was validated immunohistochemically in 50 PDAC patients, following by survival analyses and statistical comparison of protein expression with clinicopathological variables.
Paraneoplastic Ma antigen-like 1 (PNMAL1) was selected as a potential biomarker. Immunohistochemical evaluation in 50 PDAC patients revealed that its positive expression was significantly associated with the better overall survival (log-rank test; p=0.009). thus, PNMAL1 is suggested as a novel potential clinically useful prognostic biomarker of PDAC.
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[Journal Article] Twenty-one proteins up-regulated in human H-ras oncogene transgenic rat pancreas cancers are up-regulated in human pancreas cancer2013
Author(s)
Yabushita S., Fukamachi K., Kikuchi F., Ozaki M., Miyata K., Sukata T., Deguchi Y., Tanaka H., Kakehashi A., Kawamura S., Uwagawa S., Wanibuchi H., Suzui M., David B. Alexander D.B., Tsuda H.
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Journal Title
Pancreas
Volume: 42
Pages: 1034-1039
Peer Reviewed
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[Journal Article] Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors2013
Author(s)
Komatsu H., Kakehashi A., Nishiyama N., Izumi N., Mizuguchi S., Yamano S., Inoue H., Hanada S., Chung K.,Wei M., Suehiro S., Wanibuchi H.
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Journal Title
Cancer Biomarkers
Volume: 13
Pages: 171-80
Peer Reviewed
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[Journal Article] Proteome analysis based on clinicopathological significance of combined examination of CK19 and preoperative CYFRA21-1 in human lung squamous cell carcinoma2013
Author(s)
Hanada S., Nishiyama N., Mizuguchi S., Yamono S., Kakehashi A., Wei M., Inoue H., Komatsu H., Chung K., Suehiro S., Wanibuchi H.
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Journal Title
Osaka City Medical J.
Volume: 59
Pages: 35-44
Peer Reviewed
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[Presentation] Ethyl tertiary-butyl ether induces oxidative stress and 8-OHdG formation in the liver of F344 rats via activation of CAR2013
Author(s)
A. Kakehashi, A. Hagiwara, N. Imai, Y. Doi, K. Nagano, M. Banton, F. Nishimaki, H.Wanibuchi and S. Fukushima
Organizer
PXR and PPAR nuclear receptors
Place of Presentation
Gonzales Conventional Center, Texas, San-Antonio, U.S.A.
Year and Date
2013-05-12
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