2013 Fiscal Year Final Research Report
Tubulin plays important role in morphogenesis of cellular and structural atypia in papillary thyroid carcinoma tissue
| Project/Area Number |
23590412
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 甲状腺 / 乳頭癌 / 核内封入体 / 核溝 / tubulin / 核膜 / MTOC |
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| Research Abstract |
Aim of the study was to clarify a molecular background of morphogenesis of cellular and structural atypia in thyroid cancer. We established culture system, in which cultured papillary thyroid carcinoma cells artificially formed nuclear grooves (NV) and pseudonuclear inclusion (PNI). Using the culture system, we investigated expression of alpha/beta- and gamma-tubulins, nuclear membrane proteins and cytoskeletons in the cells with NV/PNI. Gamma-tubulins were detected in cytoplasm near NV and in PNI. Also, we studied expression of gamma-tubulin and nuclear membrane proteins in surgically resected human thyroid cancer tissue. Aberrant expression of gamma-tubulin distribution and nuclear membrane proteins was found in the cancer tissue. From the results, we concluded non-centrosomal microtubule organizing center and nuclear membrane proteins played a central role in NV/PNI formation in culture cells and morphogenesis of follicular and papillary structure in human cancer tissue.
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[Journal Article] Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma2012
Author(s)
Shuto M, Seyama A, Gotoh Y, Kamada K, Nakamura M, Warigaya K, Watanabe H, Ueno M, Shimizu M, Fukuda T, Murata S
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Journal Title
Acta Histochem Cytochem
Volume: 45
Pages: 25-33
Peer Reviewed
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[Journal Article] Adult nephroblastoma with predominant epithelial component : a differential diagnostic candidate of papillary renal cell carcinoma and metanephric adenoma-report of three cases
Author(s)
Watanabe S, Naganuma H, Shimizu M, Ota S, Murata S, Nihei N, Matsushima J, Mikami S, Kuroda N, Nagashima Y, Nakatani Y
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Journal Title
DOI
Peer Reviewed
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