2013 Fiscal Year Final Research Report
The molecular basis of morphological atypism (proteome analysis of KRAS downstream factors)
Project/Area Number |
23590428
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Yokohama City University |
Principal Investigator |
OKUDELA KOJI 横浜市立大学, 医学部, 講師 (10326027)
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Co-Investigator(Kenkyū-buntansha) |
KATAYA Akira 日本医科大学, 医学部, 助教 (10333113)
NAGAHARA Noriyuki 日本医科大学, 医学部, 准教授 (10208043)
WOO Tetsukan 横浜市立大学, 医学研究科, 共同研究員 (90537177)
YAZAWA Takuya 杏林大学, 医学部, 准教授 (50251054)
SHIMOYAMADA Hiroaki 杏林大学, 医学部, 助教 (60381472)
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Project Period (FY) |
2011 – 2013
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Keywords | atypism / lung cancer |
Research Abstract |
A purpose of present study is to elucidate a molecular basis of morphological atypism. This study investigated the proteome modulated by oncogenic KRAS in immortalized airway epithelial cells. We here focused on CLIC4 to investigate its potential involvement in an induction of the morphologic atypism in lung cancers. CILC4 protein levels were reduced in some lung cancer cell lines. The knockdown of CLIC4 in an immortalized airway cell line modulated a cell shape as marked enlargement of cells and nuclei. CLIC4 protein levels tended to be lower in high-grade tumors. These results suggest that the alteration in CLIC4 could induce some morphological atypism of neoplastic cells and could be involved in carcinogenesis of the lung.
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Research Products
(18 results)
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[Journal Article] Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein2014
Author(s)
Oshiro H, Fukumura H, Nagahama K, Sato I, Sugiura K, Iobe H, Okiyama E, Nagao T, Nagashima Y, Aoki I, Yamanaka S, Murakami A, Maegawa J, Chishima T, Ichikawa Y, Ishikawa Y, Nagai T, Nomura M, Ohashi K, Okudela K
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Proteome Analysis for Downstream Targets of Oncogenic KRAS - the Potential Participation of CLIC4 in Carcinogenesis in the Lung2014
Author(s)
Okudela K, Katayama A, Woo T, Mitsui H, Suzuki T, Tateishi Y, Umeda S, Tajiri M, Masuda M, Nagahara N, Kitamura H, Ohashi K
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Journal Title
PLoS One
Volume: 9
Pages: e87193
Peer Reviewed
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[Journal Article] Hepatocyte nuclear factor-1 alpha inactivated hepatocellular adenomas in patient with congenital absence of the portal vein : a case report2013
Author(s)
Tateishi Y, Furuya M, Kondo F, Torii I, Nojiri K, Tanaka Y, Umeda S, Okudela K, Inayama Y, Endo I, Ohashi K
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Journal Title
Pathol Int
Volume: 63
Pages: 358-363
Peer Reviewed
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[Journal Article] Downregulation of ALDH1A1 expression in non-small cell lung carcinomas--its clinicopathologic and biological significance2013
Author(s)
Okudela K, Woo T, Mitsui H, Suzuki T, Tajiri M, Sakuma Y, Miyagi Y, Tateishi Y, Umeda S, Masuda M, Ohashi K
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Journal Title
Int J Clin Exp Pathol
Volume: 6
Pages: 1-12
Peer Reviewed
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[Journal Article] Elevated microsatellite alterations at selected tetra-nucleotide (EMAST) in non-small cell lung cancers--a potential determinant of susceptibility to multiple malignancies2013
Author(s)
Arai H, Okudela K, Oshiro H, Komitsu N, Mitsui H, Nishii T, Tsuboi M, Nozawa A, Noishiki Y, Ohashi K, Inui K, Masuda M
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Journal Title
Int J Clin Exp Pathol
Volume: 6
Pages: 395-410
Peer Reviewed
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[Journal Article] Expression of the potential cancer stem cell markers, CD133, CD44, ALDH1, and beta-catenin, in primary lung adenocarcinoma--their prognostic significance2012
Author(s)
Okudela K, Woo T, Mitsui H, Tajiri M, Masuda M, Ohashi K
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Journal Title
Pathol Int
Volume: 62
Pages: 792-801
Peer Reviewed
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[Journal Article] Identification of miR-30d as a novel prognostic maker of prostate2012
Author(s)
Kobayashi N, Uemura H, Nagahama K, Okudela K, Furuya M, Ino Y, Ito Y, Hirano H, Inayama Y, Aoki I, Nagashima Y, Kubota Y, Ishiguro H
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Journal Title
Oncotarget
Volume: 3
Pages: 1455-71
Peer Reviewed
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[Journal Article] Pulmonary adenocarcinoma, Lambert--Eaton myasthenic syndrome, and voltage-gated calcium channels : a case report2012
Author(s)
Arai H, Inui K, Hashimoto K, Kan OK, Nishii T, Kishida H, Okudela K, Nozawa A, Kaneko T, Masuda M
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Journal Title
J Med Case Rep
Volume: 6
Pages: 281
Peer Reviewed
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