The analysis of the new inhibitory factor for cytomegalovirus in ES/iPS cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590445
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: KAWASAKI Hideya 浜松医科大学, 医学部, 助教 (90397381)
• Co-Investigator(Kenkyū-buntansha): KOSUGI Isao 浜松医科大学, 医学部, 准教授 (10252173) ; ARAI Yoshihumi 浜松医科大学, 医学部, 助教 (30381784) ; IWASHITA Toshihide 浜松医科大学, 医学部, 教授 (00283432)
• Project Period (FY): 2011 – 2013
• Keywords: ES / iPS / サイトメガロウイルス

Research Abstract

Pluripotent stem cell (iPS/ES cells) showed resistance to CMV. We established iPS cells from human fibroblast using Yamanka factors. iPS showed remarkable resistance to CMV infection. This is the compatible with previous report (Plos one: 10.1371/journal.pone.0017492). We tried to investigate the new factor for this inhibitory phenomenon.The new factor has not been found yet. Instead, we tried to find known receptor (CD29) distribution for CMV in brain. Endothelial cells, pericytes, meninges, choloid plexus and NSPC all express higher level of CD29 than other area of the brain. Co-expression of higher level of CD29 expressing cells and MCMV infected cells were highly correlated. In the brain. We concluded that cytomegalovirus infection primarily initiates infection from high-level CD29 expressing cells in acute infection phase.

Research Products

• [Journal Article] Establishment of a novel mouse model of ulcerative colitis with concomitant cytomegalovirus infection - in vivo identification of cytomegalovirus persistent infected cells (2013)
  • Author(s): Matsumura K, Nakase H, Kosugi I, Honzawa Y, Yoshino T, Matsuura M, Kawasaki H, Arai Y, Iwashita T, Nagasawa T, Chiba T
  • Journal Title: Inflammatory Bowel Disease Volume: 19(9) Pages: 1951-1956
  • Peer Reviewed
• [Journal Article] Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences (2013)
  • Author(s): Akamatsu T, Arai Y, Kosugi I, Kawasaki H, Meguro S, Sakao M, Shibata K, Suda T, Chida K, Iwashita T
  • Journal Title: Fibrogenesis Tissue Repair Volume: 6(1) Pages: 15
  • DOI: 10.1186/1755-1536-6-15
  • Peer Reviewed
• [Journal Article] An autopsy case of the marburg variant of multiple sclerosis (acute multiple sclerosis) (2013)
  • Author(s): Suzuki M, Kawasaki H, Masaki K, Suzuki S, Terada T, Tsuchida T, Tokuyama T, Kono S, Komori T, Baba S, Kira J, Miyajima H
  • Journal Title: Intern Med. Volume: 52(16) Pages: 1825-1832
  • Peer Reviewed
• [Journal Article] Expression ofβ-adrenoceptor subtypes in urothelium, interstitial cells and detrusor of the human urinary bladder (2013)
  • Author(s): Otsuka A, Kawasaki H, Matsumoto R, Shinbo H, Kurita Y, Iwashita T, Ozono S
  • Journal Title: LUTS Volume: 5 Pages: 173-180
  • Peer Reviewed
• [Journal Article] Pluripotent stem cells are protected from cytomegalovirus infection at multiple points : implications of a new pathogenesis for congenital anomaly caused by cytomegalovirus (2012)
  • Author(s): Kawasaki H
  • Journal Title: Congenit Anom Volume: 52(Review) Pages: 147-154
  • Peer Reviewed
• [Journal Article] Effects of intrapulmonary viral tropism and cytokine expression on the histological patterns of cytomegalovirus pneumonia (2012)
  • Author(s): Arai Y, Tsuchida T, Kosugi I, Kawasaki H, Meguro S, Kinoshita M, Baba S, Maeda M, Shinmura Y, Tsutsui Y, Iwashita T
  • Journal Title: Pathol Int Volume: 62 Pages: 628-639
  • Peer Reviewed
• [Journal Article] Mouse embryonic stem cells inhibit murine cytomegalovirus infection through a multi-step process (2011)
  • Author(s): Kawasaki H, Kosugi I, Arai Y, Iwashita T, Tsutsui Y
  • Journal Title: PLoS One Volume: 6 Pages: e17492
  • DOI: 10.1371
  • Peer Reviewed
• [Presentation] 急性期サイトメガロウイルス大脳感染における血行性/髄腔内感染標的細胞とintegrinβ1との関係 (2013)
  • Author(s): 河崎秀陽、鈴木万幾子、小杉伊三夫、新井義文、筒井祥博、岩下寿秀
  • Organizer: 第61回ウイルス学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-11-10
• [Presentation] 急性期サイトメガロウイルス大脳感染における血行性/髄腔内感染標的細胞とintegrinβ1との関係 (2013)
  • Author(s): 河崎秀陽、鈴木万幾子、小杉伊三夫、新井義文、筒井祥博、岩下寿秀
  • Organizer: 第102回日本病理学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-06-06
• [Presentation] サイトメガロウイルス大脳急性感染期におけるintegrinβ1発現と感染分布 (2012)
  • Author(s): 河崎秀陽、小杉伊三夫、鈴木万幾子、新井義文、筒井祥博、岩下寿秀
  • Organizer: 第52回日本先天異常学会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-06
• [Presentation] サイトメガロウイルス大脳急性感染期におけるintegrinβ1発現と感染分布 (2012)
  • Author(s): 河崎秀陽、小杉伊三夫、新井義文、筒井祥博、岩下寿秀
  • Organizer: 第101会日本病理学会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-26
• [Presentation] Mouse Embryonic Stem Cells inhibit Cyotomegalovirus Infection Through a Multi-Step Process (2011)
  • Author(s): Kawasaki H, Kosugi I, Arai Y, Iwashita T, Tsutsui Y
  • Organizer: International Congress of Virology
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-15
• [Presentation] 幹細胞に着目したサイトメガロウイルス感染感受性の解析 (2011)
  • Author(s): 河崎秀陽
  • Organizer: 第100回病理学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-04-28