Pathological analysis of chronic active EBV-infection rabbit model with hemophagocytosis

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590463
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tottori University
• Principal Investigator: HAYASHI Kazuhiko 鳥取大学, 医学部, 教授 (30180962)
• Project Period (FY): 2011 – 2013
• Keywords: EBVの感染モデル / ウサギモデル / EBNA2遺伝子欠損型EBV / ワクチン

Research Abstract

1) EBER-1 ISH revealed EBV-positive and -negative rabbits lifelong observation after EBV-inoculation.
2) EBNA-2-deleted EBV from P3HR-1 can infect rabbits with lower efficacy than prototype EBV from B85-8. However, this is the only one in vivo system to study the function of EBNA-2 gene.
3) Synthetic peptides of Epstein-Barr Virus-major envelope glycoprotein-350/220 do not prevent infection in a rabbit Epstein-Barr Virus infection model.

Research Products

• [Journal Article] Hayashi EBNA-2-deleted Epstein–Barr virus from P3HR-1 can infect rabbits with lower efficiency than prototype Epstein–Barr virus from B95-8 (2013)
  • Author(s): H. Sano, K. Nagata, K. Kato, K. Kanai, K. Yamamoto, K. Okuno, S. Kuwamoto, H. Higaki-Mori, H. Sugihara, M. Kato, I. Murakami, S. Kanzaki, K
  • Journal Title: Intervirology Volume: 56 Pages: 114-121
  • Peer Reviewed
• [Journal Article] EBウイルスと甲状腺疾患成人病と生活習慣病 (2013)
  • Author(s): 長田佳子,林一彦
  • Volume: 43
  • Pages: 1103-1107
• [Journal Article] Synthetic Peptides of Epstein-Barr Virus-major envelope glycoprotein-350/220 Do Not Prevent Infection in a Rabbit Epstein-Barr Virus Infection Model (2012)
  • Author(s): Kaoru Kato, Hitoshi Sano, Keiko Nagata, Hirotsugu Sugihara, Kyosuke Kanai, Satoshi Kuwamoto, Masako Kato, Ichiro Murakami, Kazuhiko Hayashi
  • Journal Title: J Vaccine & Vaccination Volume: 4 Pages: 2157-2160
  • Peer Reviewed
• [Journal Article] In Vitro EBV infection Model of Rabbit Lymphocytes from Peripheral Blood or Spleen (2011)
  • Author(s): Kyosuke Kanai, Kaoru Kato, Hitoshi, Sano, Keiko Nagata, Keisuke Okuno, Satoshi Kuwamoto, Hiromi Higaki, Hirotsugu Sugihara, Masako Kato, Ichiro Murakami, Kazuhiko Hayashi
  • Journal Title: Intervirology Volume: 54 Pages: 17-24
  • Peer Reviewed
• [Journal Article] A rabbit model for primary and lifelong persistent Epstein-Barr virus infection (2011)
  • Author(s): Hayashi K, K Takashima, K Okuno, K. Kato, S Sano, H Sugihara, M Kato, I Murakami K, K Nagata
  • Journal Title: Virchows Arch Volume: 459(Suppl 1) Pages: S302
  • Peer Reviewed
• [Presentation] EBV関連遺伝子mRNA発現の定量法の検討 (2013)
  • Author(s): 越智万梨華、長田佳子、松下倫子、佐藤幸夫、林一彦
  • Organizer: 第10会EBウイルス研究会
  • Place of Presentation: キャンパスプラザ京都
  • Year and Date: 2013-07-12
• [Presentation] EBウイルス感染B細胞株の再活性化後期におけるEBERとgp350/ 220の局在:72A1抗体を用いたP3HR-1細胞株の蛍光染色 (2012)
  • Author(s): 長田佳子、檜垣克美、中山祐二、金井亨輔、桐谷唯、佐野仁志、加藤郁、木村宏、西蓮寺剛、林一彦
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 大阪国際会議場(大阪府
  • Year and Date: 20121113-15
• [Presentation] バセドウ病患者の末梢血におけるEBV loadの検討 (2012)
  • Author(s): 桐谷唯、長田佳子、木村宏、石黒清介、佐野仁志、加藤郁、林一彦
  • Organizer: 第9会EBウイルス研究会
  • Place of Presentation: 米子市文化ホール(鳥取県)
  • Year and Date: 2012-07-06
• [Presentation] A rabbit model for primary and lifelong persistent Epstein-Barr virus infection (2011)
  • Author(s): Hayashi K, K Takashima, K Okuno, K. Kato, S Sano, H Sugihara, M Kato, I Murakami K, K Nagata
  • Organizer: 23rd European Congress of Pathology
  • Place of Presentation: Helsinki Exhibition & Convention Centre, Helsinki, Finland
  • Year and Date: 2011-08-31
• [Presentation] P3HR-1株産生EBVの経鼻投与におけるEBウイルス感染ウサギの解析 (2011)
  • Author(s): 佐野仁志、金井亨輔、加藤郁、長田佳子、林一彦
  • Organizer: 第8回EBウイルス研究会
  • Place of Presentation: 大阪大学大学院銀杏会館(大阪)
  • Year and Date: 2011-07-08