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2013 Fiscal Year Final Research Report

Regulation of pathogenicity via envelope stress response in Bordetella pertussis

Research Project

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Project/Area Number 23590517
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyorin University

Principal Investigator

HANAWA Tomoko  杏林大学, 医学部, 講師 (80255405)

Co-Investigator(Kenkyū-buntansha) KAMIYA Shigeru  杏林大学, 医学部, 教授 (10177587)
Co-Investigator(Renkei-kenkyūsha) YONEZAWA Hideo  杏林大学, 医学部, 講師 (60453528)
YAMAMOTO Tomoko  千葉大学, 薬学研究科, 教授 (60110342)
Research Collaborator ARMSTRONG Sandra K  ミネソタ大学, 医学研究科, 教授
Project Period (FY) 2011 – 2013
Keywords百日咳菌 / 細胞表層 / ストレス応答 / 病原性 / sigmaE
Research Abstract

Cell surface has an important role in the functioning of Bordetella pertussis virulence factors. We found cell-free adenylate cyclase toxin (ACT) was increased by the activation of sigma E, which is alternative sigma subunit of RNA polymerase relating to envelope stress response. In the present study, we investigated the mechanism of increase of cell-free ACT by sigma E activation.
Transcriptions of the genes encoding ACT and its secretion machinery did not altered by activation of sigma E. On the other hand, production of outer membrane vesicle (OMV) including ACT was significantly increased. Different manner of release would provide the stability to ACT resulted in the increase of cell-free ACT. ACT inhibits phagocytosis by macrophages. Activation of sigma E by deletion of RseA was decreased the efficiency of phagocytosis. It is suggested that sigma E-dependent envelope stress response would be one of the regulation system of the pathogenicity of B. pertussis.

  • Research Products

    (8 results)

All 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Role of Bordetella pertussis RseA in the cell envelope stress response and adenylate cyclase toxin release2013

    • Author(s)
      Tomoko Hanawa, Hideo Yonezawa, Hayato Kawakami, Shigeru Kamiya, Sandra Armstrong
    • Journal Title

      Pathog Dis

      Volume: 69 Pages: 7-20

    • DOI

      10.1111/2049-632X.12061

    • Peer Reviewed
  • [Journal Article] Role of (p)ppGpp in biofilm formation and expression of filamentous structures in Bordetella pertussis2013

    • Author(s)
      Kentaro Sugisaki, Tomoko Hanawa, Hideo Yonezawa, Takako Osaki, Toshiyuki Fukutomi, Kawakami H, Tomoko Yamamoto, Shigeru Kamiya
    • Journal Title

      Microbiology

      Volume: 159(7) Pages: 1379-1389

    • DOI

      10.1099/mic.0.066597-0

    • Peer Reviewed
  • [Presentation] Expression of virulence factors under nutrient-limited condition in Bordetella pertussis2013

    • Author(s)
      Tomoko Hanawa, Kentaro Sugisaki, Hideo Yonezawa, Hayato Kawakami, Shigeru Kamiya
    • Organizer
      10th International Symposium on Bordetella
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      20130908-11
  • [Presentation] 百日咳菌(p)ppGpp欠損株のBiofilm形成および病原因子の発現2013

    • Author(s)
      杉崎健太郎,花輪智子,米澤英雄,大﨑敬子,蔵田訓,北条史,神谷茂
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Year and Date
      20130318-20
  • [Presentation] Biofilm formation of Bordetella pertussis; response to nutritional starvation2012

    • Author(s)
      Tomoko Hanawa, Kentaro Sugisaki, Hideo Yonezawa, Hayato Kawakami, Shigeru Kamiya
    • Organizer
      Biofilms 5
    • Place of Presentation
      Paris, France
    • Year and Date
      20121210-12
  • [Presentation] Implication of the role of stringent response in the expression of adenylate cyclase toxin in Bordetella pertussis2011

    • Author(s)
      Tomoko Hanawa, Kentrao Sugisaki, Hideo Yonezawa, Takako Osaki, Satoshi Kurata, Cynthia Zaman, Shigeru Kamiya
    • Organizer
      The Joint Meeting of the 17th International Symposium on Gnotobiology (ISG) and the 34th Congress of Society for Microbial Ecology and Disease
    • Place of Presentation
      Yokohama
    • Year and Date
      20111120-23
  • [Presentation] Envelope stress response and secretion in Bordetella pertussis2011

    • Author(s)
      Tomoko Hanawa, Hideo Yonezawa, Takako Osaki, Satoshi Kurata, Cynthia Zaman, Sandra Armstrong, and Shigeru Kamiya
    • Organizer
      XIII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo
    • Year and Date
      20110906-10
  • [Presentation] Role ofσ^E in the envelope stress response and an implication for the production of toxins in Bordetella pertussis2011

    • Author(s)
      Tomoko Hanawa, Hideo Yonezawa, Shigeru Kamiya, Sandra Armstrong
    • Organizer
      111th general meeting of the American Society for Microbiology
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      20110521-24

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Published: 2015-07-16  

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