2013 Fiscal Year Final Research Report
Cellular mechanism of toxicity of Clostridium perfringens iota-toxin
| Project/Area Number |
23590526
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | Tokushima Bunri University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
永浜 政博 (40164462)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | ウエルシュ菌イオタ毒素 / エンドサイトーシス / 初期エンドソーム / リサイクリングエンドソーム / 後期エンドソーム / リソソーム |
|---|
| Research Abstract |
Clostridium perfringens iota-toxin is a binary toxin composed of an enzyme component(Ia) and a binding component(Ib). Each component alone lacks toxic activity, but together they produce cytotoxic effects. This study indicated that an internalized Ia and Ib complex was delivered to early endosomes and that subsequent delivery of Ia to the cytoplasm occured mainly in early endosomes. Then, Ib was transported to late endosomes and lysosomes for degradation. Next, we examined the cytotoxicity of Ib. A431 and A549 cells were susceptible to Ib. Ib bound and formed oligomers in the membranes of A431 cells. Then, Ib caused cell swelling and the rapid depletion of cellular ATP in the cells. Ib also induced permeabilization by propidium iodide without DNA fragmentation in the cells. Ultrastructural studies revealed that A431 cells underwent necrosis after treatment with Ib. We demonstrated that Ib by itself produced cytotoxic activity through necrosis.
|
-
-
-
[Journal Article] Clostridium perfringens alpha-toxin induces the release of IL-8 through a dual pathway via TrkA in A549 cells2012
Author(s)
Masataka Oda, Ryota Shiihara, Yuka Ohmae, Michiko Kabura, Teruhisa Takagishi, Keiko Kobayashi, Masahiro Nagahama, Masahisa Inoue, Tomomi Abe, Koujun Setsu, Jun Sakurai
-
Journal Title
Biochim. Biophys. Acta
Volume: 1822(10)
Pages: 1581-1589
-
-
[Journal Article] Clostridium perfringens alpha-toxin recognizes the GM1a-Trk A complex2012
Author(s)
Masataka Oda, Michiko Kabura, Teruhisa Takagishi, Ayaka Suzue, Kaori Tominaga, Shiori Urano, Masahiro Nagahama, Keiko Kobayashi, Keiko Furukawa, Koichi Furukawa, Jun Sakurai
-
Journal Title
J . Biol. Chem
Volume: 287(39)
Pages: 33070-33079
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
