2013 Fiscal Year Final Research Report
Analysis for role of bacterial cytoskeleton RodZ on virulence gene expression of Shigella type III secretion system
| Project/Area Number |
23590531
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
MITOBE Jiro 国立感染症研究所, 細菌第一部, 主任研究官 (40333364)
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| Co-Investigator(Kenkyū-buntansha) |
YANAGIHARA Itaru 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), 免疫部門, 部長 (60314415)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 病原性 / バクテリア細胞骨格 / 転写後制御 / RNA結合蛋白 / III型分泌装置 / 赤痢菌 |
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| Research Abstract |
Bacterial cytoskeleton RodZ was isolated as a factor affecting virulence gene expression of Shigella sonnei. Messenger RNA of the virulence gene was highly stabilized in the deletion mutant of rodZ gene and consistently purified RodZ protein bound to the RNA probe. Finding of amino-acids cluster essential for RNA binding indicated that RodZ has two distinct functions for RNA binding and cell-shape formation. We also found the membrane protein RodZ existed as homohexamer similar to another RNA binding protein Hfq.
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