2013 Fiscal Year Final Research Report
Molecular mechanism of the paramyxovirus-mediated membrane fusion as analyzed by novel procedures for detection
| Project/Area Number |
23590538
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Mie University |
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Principal Investigator |
TSURUDOME Masato 三重大学, 医学(系)研究科(研究院), 准教授 (50159042)
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| Project Period (FY) |
2011 – 2013
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| Keywords | パラインフルエンザウイルス / ＨＮ蛋白 / Ｆ蛋白 / 分子間相互作用 / 膜融合 |
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| Research Abstract |
Chimeric analysis of the F proteins of the parainfluenza virus 5 and simian virus 41 revealed that 21 amino acids in the five domains on the surface of the F protein head region are involved in functional interaction with the HN protein stalk region, leading to the induction of cell-cell fusion. Furthermore, it was suggested that this functional interaction is dependent on the conformation of the HN stalk region.
On the other hand, the results obtained from bimolecular fluorescence complementation analysis indicated that physical interaction between HN and F protein is not only dependent on the proteolytic cleavage of the F protein but also on the conformational change of the F protein. However, it was suggested that under physiological conditions his physical interaction is weak in its avidity or takes place very transiently.
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Author(s)
Kihira S,Uematsu J,Ishitana Y,Chindoh M,Baba A,Kuzuta R,Hasegawa T,Fujimoto K,Funauchi A,Yamamoto H,Kawano M,Tsurudome M,O'Brien M,Komada H
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Int. J. Sci
Volume: 3
Pages: 14-20
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Author(s)
Kitagawa H,Kawano M,Yamanaka K,Kakeda M,Tsuda K,Inada H,Yoneda M,Sakaguchi T,Nigi A,Nishimura K,Komada H,Tsurudome M,Yasutomi Y,Nosaka T,Mizutani H
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Journal Title
PLoS One
Volume: 8
Pages: e66614
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[Journal Article] Legume lectins inhibit human parainfluenza virus type 2 infection by interfering with the entry2012
Author(s)
Uematsu J,Koyama A,Takano S,Ura Y,Tanemura M,Kihira S,Yamamoto H,Kawano M,Tsurudome M,O'Brien M,Komada H
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Journal Title
Viruses
Volume: 4
Pages: 1104-1115
Peer Reviewed
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[Journal Article] Completion of the full-length genome sequence of human parainfluenza virus types 4A and 4B : sequence analysis of the large protein genes and gene start,intergenic and end sequences2011
Author(s)
Komada H,Kawano M,Uefuji A,Ito M,TsurudomeM,Hatakeyama E,Nakanishi M,Sakue S,Joh C,Suzumura E,Tamaki T,Tomioka T,Nishio M,Tsumura H,Uematsu J,Yamamoto H,O'Brien M,Bando H,Ito
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Journal Title
Arch. Virol
Volume: 156
Pages: 161-166
Peer Reviewed
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[Presentation]2012
Author(s)
山本秀孝,紀平佐保子,植松淳,河野光雄,鶴留雅人,駒田洋
Organizer
日本社会薬学会第31年会
Place of Presentation
鈴鹿
Year and Date
2012-09-16
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