2013 Fiscal Year Final Research Report
Analysis of virus replication mechanism using minigenome or recombinant virus system
| Project/Area Number |
23590539
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
NISHIO Machiko 和歌山県立医科大学, 医学部, 教授 (70156040)
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| Project Period (FY) |
2011 – 2013
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| Keywords | パラインフルエンザウイルス２型 / リコンビナントウイルス / ミニゲノムシステム / V蛋白 / ウイルス増殖機構 / caspase-1 / tetherin |
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| Research Abstract |
To search for host factors interacting with human parainfluenza virus type 2 (hPIV2) V protein systematically, I used the yeast two-hybrid system, and identified some proteins. Tetherin is a cellular membrane protein that restricts budding of a number of enveloped viruses. I demonstrated that tetherin interacts with V protein, and inhibits hPIV2 growth. The V protein inhibits the caspase-1-mediated maturation of IL-1beta via its interaction with caspase-1. Moreover, it interacts with adaptor-related protein complex 1 (AP-1) that is important for apical sorting of hPIV2.
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