2013 Fiscal Year Final Research Report
Analysis of Restriction Factors of cynomolgus monkey in establishment of HIV-1/monkey model
| Project/Area Number |
23590541
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAYAMA Emi 大阪大学, 微生物病研究所, 准教授 (70324845)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ヒト免疫不全ウイルス / 感染抑制因子 / 動物モデル |
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| Research Abstract |
TRIM5alpha was previously identified as an intrinsic factor that restricts HIV-1 replication in rhesus monkey cells. Thereafter, TRIMCyp was discovered as a mutated allele of TRIM5 gene. We demonstrated that the frequencies of TRIMCyp in cynomolgus monkey markedly higher than that of rhesus monkey. Then, we infected monkey-tropic HIV-1 (MN4Rh-3) to TRIMCyp homozygotes or TRIM5alpha homozygotes. The viral growth was higher in TRIMCyp homozygotes than that in TRIM5alpha homozygotes. These results indicated that genotyping of TRIM5 gene is important to select monkey susceptible to monkey-tropic HIV-1 in establishment of HIV-1/monkey model system.
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[Journal Article] Generation of rhesus macaque-tropic HIV-1 clones that are resistant to major anti-HIV-1 restriction factors2013
Author(s)
Nomaguchi M, Yokoyama M, Kono K, Nakayama EE, Shioda T, Doi N, Fujiwara S, Saito A, Akari H, Miyakwa K, Ryo A, Ode H, Iwatani Y, Miura T, Igarashi T, Sato H, Adachi A
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Journal Title
J Virol
Volume: 87
Pages: 11447-11461
DOI
Peer Reviewed
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[Journal Article] TRIM5 genotypes in cynomolgus monkeys primarily influence inter-individual diversity in susceptibility to monkey-tropic human immunodeficiency virus type 12013
Author(s)
Saito A, Nomaguchi M, Kono K, Iwatani Y, Yokoyama M, Yasutomi Y, Sato H, Shioda T, Sugiura W, Matano T, Adachi A, Nakayama EE, Akari H
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Journal Title
J Gen Virol
Volume: 94
Pages: 1318-1324
DOI
Peer Reviewed
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[Journal Article] Polymorphisms in Fas gene is associated with HIV-related lipoatrophy in Thai patients2013
Author(s)
Likanonsakul S, Rattanatham T, Feangvad S, Uttayamakul S, Prasithsirikul W, Srisopha S, Nitiyanontakij R, Tengtrakulcharoen P, Tarkowski M, Riva A, Nakayama EE, Shioda T
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Journal Title
AIDS Res Hum Retroviruses
Volume: 29
Pages: 142-150
DOI
Peer Reviewed
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[Journal Article] The cauboxyl-terminus of human immunodeficiency virus type 2 circulating recombinant form 01_AB capsid protein affects sensitivity to human TRIM5α2012
Author(s)
Miyamoto T, Nakayama EE, Yokoyama M, Ibe S, Takehara S, Kono K, Yokomaku Y, Pizzato M, Luban J, Sugiura W, Sato H, Shioda T
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Journal Title
PLoS ONE
Volume: 7
Pages: e47757
DOI
Peer Reviewed
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