Study on regulatory mechanism of RNA viral genome by cellular bodies

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590545
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Kumamoto University
• Principal Investigator: ARIUMI YASUO 熊本大学, エイズ学研究センター, 准教授 (60303913)
• Project Period (FY): 2011 – 2013
• Keywords: HCV / P-pody / ストレス顆粒 / RNAヘリケース / APOBEC3G / HIV-1

Research Abstract

We identified stress granule components as the hepatitis C virus (HCV) genome regulatory proteins. Notably, we noticed that HCV infection induces stress granules 36h post-infection. Furthermore, HCV hijacked these stress granule components, including G3BP1, ataxin-2, poly(A)-binding protein (PABP)-1, around lipid droplets. Importantly, HCV replication was significantly suppressed in these knockdown cells. Thus, we suggested that stress granule components are required for HCV replication.

Research Products

• [Journal Article] Adenosine kinase is a key determinant for the anti-HCV activity of ribavirin (2013)
  • Author(s): Mori K, Hiraoka O, Ikeda M, Ariumi Y, Hiramoto A, Wataya Y, Kato N
  • Journal Title: Hepatology Volume: 58 Pages: 1236-1244
  • Peer Reviewed
• [Journal Article] PML tumore suppressor protein is required for HCV production (2013)
  • Author(s): Kuroki M, Ariumi Y, Hijikata M, Ikeda M, Dansako H, Wakita T, Shimotohno K, Kato N
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 430 Pages: 592-597
  • Peer Reviewed
• [Journal Article] DDX3 RNA helicase is required for HIV-1 Tat function (2013)
  • Author(s): Yasuda-Inoue M, Kuroki M, Ariumi Y
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 441 Pages: 607-611
  • Peer Reviewed
• [Journal Article] Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev function (2013)
  • Author(s): Yasuda-Inoue M, Kuroki M, Ariumi Y
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 434 Pages: 803-808
  • Peer Reviewed
• [Journal Article] Distinct HIV-1 escape patterns selected by CTLs with identical epitope specificity (2013)
  • Author(s): Yagita Y, Kuse N, Kuroki K, Gatanaga H, Carlson J, Chikata T, Brumme Z, Murakoshi H, Akahoshi T, Pfeifer N, Mallal S, John M, Ose T, Matsubara H, Kanda R, Fukunaga Y, Honda K, Kawashima Y, Ariumi Y, Oka S, Maenaka K, Takiguchi M
  • Journal Title: J. Virol Volume: 87 Pages: 2253-2263
  • Peer Reviewed
• [Journal Article] Identification of host genes showing differential expression profiles with cell-based long-term replication of hepatitis C virus RNA (2012)
  • Author(s): Sejima H, Mori K, Ariumi Y, Ikeda M, Kato N
  • Journal Title: Virus Res Volume: 167 Pages: 74-85
  • Peer Reviewed
• [Journal Article] Development of hepatitis C virus production reporter-assay systems using two different hepatoma cell lines (2012)
  • Author(s): Takeda M, Ikeda M, Ariumi Y, Wakita T, Kato N
  • Journal Title: J. Gen. Virol Volume: 93 Pages: 1422-1431
  • Peer Reviewed
• [Journal Article] Raloxifene inhibits hepatitis C virus infection and replication (2012)
  • Author(s): Takeda M, Ikeda M, Mori K, Yano M, Ariumi Y, Dansako H, Wakita T, Kato N
  • Journal Title: FEBS Open Bio Volume: 22 Pages: 279-283
  • Peer Reviewed
• [Journal Article] Identification of the P-body component PATL1 as a novel ALG-2-intercating protein by in silico and far-Western screening of proline-rich proteins (2012)
  • Author(s): Osugi K, Suzuki H, Nomura T, Ariumi Y, Shibata H, Maki M
  • Journal Title: J. Biochem Volume: 151 Pages: 657-666
  • Peer Reviewed
• [Journal Article] Development of a drug assay system with hepatitis C virus genome derived from a patient with acute hepatitis C (2012)
  • Author(s): Mori K, Ueda Y, Ariumi Y, Dansako H, Ikeda M, Kato N
  • Journal Title: Virus Genes Volume: 44 Pages: 374-381
  • Peer Reviewed
• [Journal Article] Hepatitis C virus hijacks P-body and stress granule components around lipid droplets (2011)
  • Author(s): Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, Ikeda M, Kato N
  • Journal Title: J Virol Volume: 85(14) Pages: 6882-6892
  • Peer Reviewed
• [Journal Article] Mechanism of action of ribavirin in a novel hepatitis C virus replication cell system (2011)
  • Author(s): Mori K, Ikeda M, Ariumi Y, Dansako H, Wakita T, Kato N
  • Journal Title: Virus Res Volume: 157(1) Pages: 61-70
  • Peer Reviewed
• [Journal Article] Plural assay systems derived from different cell lines and hepatitis C virus strains are required for the objective evaluation of anti-hepatitis C virus reagents (2011)
  • Author(s): Ueda Y, Mori K, Ariumi Y, Ikeda M, Kato N
  • Journal Title: Biochem Biophys Res Commun Volume: 409(4) Pages: 663-668
  • Peer Reviewed
• [Journal Article] Anti-ulcer agent teprenone inhibits hepatitis C virus replication : potential treatment for hepatitis C (2011)
  • Author(s): Ikeda M, Kawai Y, Mori K, Yano M, Abe K, Nishimura G, Dansako H, Ariumi Y, Wakita T, Yamamoto K, Kato N
  • Journal Title: Liver Int Volume: 31(6) Pages: 871-880
  • Peer Reviewed
• [Journal Article] HCVによる肝発癌機構 (2011)
  • Author(s): 有海康雄、加藤宣之
  • Journal Title: 日本臨床(日本臨床社)(増刊号新時代のウイルス性肝炎学—基礎•臨床研究の進歩—) Volume: 69巻、増刊4号 Pages: 64-68
• [Presentation] Host factors involved in LINE-1 retrotransposition (2014)
  • Author(s): Yasuo Ariumi, Misao Kuroki
  • Organizer: Keystone symposia Mobile Genetic Elements and Genome Evolution (C2)
  • Place of Presentation: Santa Fe, New Mexico, USA
  • Year and Date: 20140309-14
• [Presentation] HIV-1 interacts with LINE-1 (2014)
  • Author(s): Kodai Kawano, Misao Kuroki, Yasuo Ariumi
  • Organizer: Keystone symposia Mobile Genetic Elements and Genome Evolution (C2)
  • Place of Presentation: Santa Fe, New Mexico, USA
  • Year and Date: 20140309-14
• [Presentation] P-body因子によるRNAウイルス、寄生ゲノムの制御機構 (2014)
  • Author(s): 有海康雄
  • Organizer: 東京大学医科学研究所第2回感染症国際研究センターシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2014-03-18
• [Presentation] がん抑制因子はLINE-1のレトロトランスポジションを抑制する (2013)
  • Author(s): 黒木美沙緒、井上万里子、中島詩織、有海康雄
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 20131110-12
• [Presentation] HIV-1によるLINE-1レトロトランスポジションの抑制 (2013)
  • Author(s): 川野広大、黒木美沙緒、有海康雄
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 20131110-12
• [Presentation] P-body, RNA helicase, and HIV-1 life cycle (2013)
  • Author(s): Yasuo Ariumi
  • Organizer: 14th Kumamoto AIDS Seminar
  • Place of Presentation: 阿蘇リゾートグランヴィリオホテル、Kumamoto, Japan
  • Year and Date: 20131029-31
• [Presentation] HIV-1 suppresses LINE-1 retrotransposition (2013)
  • Author(s): Kodai Kawano, Misao Kuroki, Yasuo Ariumi
  • Organizer: 14th Kumamoto AIDS Seminar
  • Place of Presentation: 阿蘇リゾートグランヴィリオホテル、Kumamoto, Japan
  • Year and Date: 20131029-31
• [Presentation] レトロエレメントの転移と宿主因子 (2013)
  • Author(s): 有海康雄
  • Organizer: 国立遺伝学研究所研究集会「転移因子と宿主の相互作用による生命機能」
  • Place of Presentation: 国立遺伝学研究所、三島、静岡
  • Year and Date: 20131010-11
• [Presentation] Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev and Tat function (2013)
  • Author(s): Yasuo Ariumi, Mariko Yasuda-Inoue, Misao Kuroki
  • Organizer: Frontiers of retrovirology
  • Place of Presentation: Churchill College, Cambridge University, UK
  • Year and Date: 20130916-18
• [Presentation] Tumor suppressor proteins restricts LINE-1 retrotransposition (2013)
  • Author(s): Misao Kuroki, Mariko Yasuda-Inoue, Shiori Nakashima, Yasuo Ariumi
  • Organizer: Frontiers of retrovirology
  • Place of Presentation: Churchill College, Cambridge University, UK
  • Year and Date: 20130916-18
• [Presentation] Tumor suppressor proteins negatively regulates the life cycle of LINE-1 (2013)
  • Author(s): Yasuo Ariumi and Misao Kuroki
  • Organizer: FASEB meeting Mobile DNA in mammalian genomes
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 20130609-14
• [Presentation] Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev and Tat function (2013)
  • Author(s): Yasuo Ariumi, Mariko Yasuda-Inoue, Misao Kuroki
  • Organizer: Cold Spring Harbor Rettoviruses Meeting
  • Place of Presentation: Cold Spring Harbor, New York, USA
  • Year and Date: 20130520-25
• [Presentation] Identification and characterization of novel restriction factors of LINE-1 (2013)
  • Author(s): Misao Kuroki, Mariko Yasuda-Inoue, Shiori Nakashima, Yasuo Ariumi
  • Organizer: Cold Spring Harbor Rettoviruses Meeting
  • Place of Presentation: Cold Spring Harbor, New York, USA
  • Year and Date: 20130520-25
• [Presentation] P-body因子MOV10はHIV-1複製とLINE-1のレトロトランスポジションを抑制する (2012)
  • Author(s): 黒木美沙緒、井上万里子、有海康雄
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 20121211-14
• [Presentation] P-body因子MOV10はHIV-1複製とLINE-1のレトロトランスポジションを抑制する (2012)
  • Author(s): 黒木美沙緒、井上万里子、有海康雄
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪、大阪
  • Year and Date: 20121113-15
• [Presentation] DDX DEAD-box RNA helicase familyによるHIV-1複製調節タンパクRev、Tatの機能制御 (2012)
  • Author(s): 井上万里子、黒木美沙緒、有海康雄
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪、大阪
  • Year and Date: 20121113-15
• [Presentation] P-bodyとHCV-1のクロストーク (2012)
  • Author(s): 有海康雄、黒木美沙緒、井上万里子、土方誠、池田正徳、脇田隆字、下遠野邦忠、加藤宣之
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪、大阪
  • Year and Date: 20121113-15
• [Presentation] The P-body component MOV10 inhibits HIV-1 replication and LINE-1 retrotransposition (2012)
  • Author(s): Misao Kuroki, Mariko Inoue, Yasuo Ariumi
  • Organizer: 13th Kumamoto AIDS Seminar Global COE Joint International Symposium
  • Place of Presentation: 阿蘇リゾートグランヴィリオホテル、熊本
  • Year and Date: 20121024-26
• [Presentation] DDX DEAD-box RNA helicase family modulates HIV-1 Rev and Tat function (2012)
  • Author(s): Mariko Inoue, Misao Kuroki, Yasuo Ariumi
  • Organizer: 13th Kumamoto AIDS Seminar Global COE Joint International Symposium
  • Place of Presentation: 阿蘇リゾートグランヴィリオホテル、熊本
  • Year and Date: 20121024-26
• [Presentation] Dynamic regulation of cytoplasmic mRNA-containing bodies in HCV systems (2012)
  • Author(s): Yasuo Ariumi, Misao Kuroki, Mariko Inoue, Makoto Hijikata, Masanori Ikeda, Takaji Wakita, Kunitada Shimotohno, Nobuyuki Kato
  • Organizer: 19th international symposium on hepatitis C virus and related viruses
  • Place of Presentation: ベニス、イタリア
  • Year and Date: 20121005-09
• [Presentation] Can P-body associated host factors APOBEC3G and MOV10 restrict HCV infection? (2012)
  • Author(s): Misao Kuroki, Mariko Inoue, Makoto Hijikata, Masanori Ikeda, Takaji Wakita, Kunitada Shimotohno, Nobuyuki Kato, Yasuo Ariumi
  • Organizer: 19th international symposium on hepatitis C virus and related viruses
  • Place of Presentation: ベニス、イタリア
  • Year and Date: 20121005-09
• [Presentation] Hepatitis C virus hijacks P-body and stress granule components around lipid droplets (2011)
  • Author(s): Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, Ikeda M, Wakita T, Kato N
  • Organizer: 18th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Seattle, U. S. A.
  • Year and Date: 20110908-12
• [Presentation] HCV production requires the PML tumor suppressor protein (2011)
  • Author(s): Kuroki M, Ariumi Y, Ikeda M, Dansako H, Wakita T, Kato N
  • Organizer: 18th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Seattle, U. S. A.
  • Year and Date: 20110908-12