Research in anti-retroviral intrinsic immunity using APOBEC1 knock-out mice

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590546
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Kumamoto University
• Principal Investigator: KOITO ATSUSHI 熊本大学, 大学院生命科学研究部, 研究員 (70231305)
• Project Period (FY): 2011 – 2013
• Keywords: レトロウイルス / シチジン脱アミノ化酵素 / レトロエレメント / HIV / 小動物モデル / ゲノム

Research Abstract

The ability of mammalian cytidine deaminases encoded by the APOBEC3 (A3) genes to restrict a broad range of endogenous retroelements as well as exogenous retroviruses such as HIV-1 is now well established. We observed that APOBEC1, which mediates C-to-U deamination of apo B mRNA and is involved in lipid transport, from rodents and non-placental mammals, such as opossum are also powerful inhibitors of retroelements including HIV-1. Accordingly, we will test the hypothesis that APOBEC1 knock-out to evade species-specific intrinsic restriction factors in mice will markedly increase their susceptibility to HIV-1 challenge.

Research Products

• [Journal Article] GANP interacts with APOBEC3G and facilitates its encapsidation into the virions to reduce HIV-1 infectivity (2013)
  • Author(s): Maeda K, Almofty SA, Singh SK, Eid MMA, Shimoda M, Ikeda T, Koito A, Pham P, Goodman MF, and Sakaguchi N.
  • Journal Title: J. Immunol Volume: 191 Pages: 6030-6039
  • DOI: 10.4049/jimmunol.1302057
  • Peer Reviewed
• [Journal Article] Intrinsic immunity by APOBEC family of cytidine deaminases against retrotransposons, in Sequence and Genome Analysis II–Bacteria (2013)
  • Author(s): Koito A.
  • Journal Title: Viruses and Metabolic Pathways (ed. iConcept Press Ltd) Pages: 96-124
  • Peer Reviewed
• [Journal Article] Retroviruses, retroelements and their restrictions (2013)
  • Author(s): Koito A, and Ishizaka Y.
  • Journal Title: Front. Microbiol Volume: 4 Pages: 197
  • DOI: 10.3389/fmicb.2013.00197
  • Peer Reviewed
• [Journal Article] Intrinsic immunity against retrotransposons by APOBEC cytidine deaminases (2013)
  • Author(s): Koito A, Ikeda T.
  • Journal Title: Front. Microbiol Volume: 4 Pages: 28
  • DOI: 10.3389/fmicb.2013.00028
  • Peer Reviewed
• [Journal Article] Apolipoprotein B mRNA-editing, catalytic polypeptide cytidine deaminases and retroviral restriction (2012)
  • Author(s): Koito A, and Ikeda T.
  • Journal Title: Wiley Interdisciplinary Reviews (WIREs) RNA Volume: 3 Pages: 529-541
  • DOI: 10.1002/wrna.1117
  • Peer Reviewed
• [Journal Article] Intrinsic restriction activity by AID/APOBEC family of enzymes against the mobility of retroelements (2011)
  • Author(s): Koito A, and Ikeda T.
  • Journal Title: Mobile Genet. Elem Volume: 1 Pages: 197-202
  • DOI: 10.4161/mge.1.3.17430
  • Peer Reviewed
• [Journal Article] Vipirinin, a coumarin-based HIV-1 Vpr inhibitor, interacts with a hydrophobic region of Vpr (2011)
  • Author(s): Ong EBB, Watanabe N, Saito A, Futamura Y, Abd El Galil KH, Koito A, Najimudin N, and Osada H.
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 14049-14056
  • DOI: 10.1074/jbc.M110.185397
  • Peer Reviewed
• [Journal Article] Intrinsic restriction activity by apolipoprotein B mRNA editing enzyme APOBEC1 against the mobility of autonomous retrotransposons (2011)
  • Author(s): Ikeda T, Abd El Galil, KH, Tokunaga K, Maeda K, Sata T, Sakaguchi N, Heidmann T, and Koito A.
  • Journal Title: Nucleic Acids Res Volume: 39 Pages: 5538-5554
  • DOI: 10.1093/nar/gkr124
  • Peer Reviewed
• [Presentation] HIV感染経路における宿主因子GANPの役割 (2013)
  • Author(s): 前田和彦、Almofty SA, Singh SK, Eid MMA,下田真唯子,川畑輝哉,池田輝政、小糸厚、坂口薫雄
  • Organizer: 第27回日本エイズ学会学術集会
  • Place of Presentation: 市民会館崇城大学ホール(熊本)
  • Year and Date: 20131120-22
• [Presentation] Role of mammalian TREX-2 component GANP in HIV-1 infection (2013)
  • Author(s): Maeda K, Almofty SA, Singh SK, Eid MMA, Shimoda M, Ikeda T, Shida H, Koito A, Pham P, Goodman MF, and Sakaguchi N.
  • Organizer: 14th. Kumamoto AIDS Seminar
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 20131029-31
• [Presentation] GANPを介したAPOBEC3Gの抗HIV感染阻止機構の解析 (2012)
  • Author(s): 前田和彦、Almofty SA, Singh SK, Eid MMA,下田真唯子,池田輝政、小糸厚、坂口薫雄
  • Organizer: 第26回日本エイズ学会学術集会
  • Place of Presentation: 慶応義塾大学日吉キャンパス(横浜)
  • Year and Date: 20121124-26
• [Presentation] 有袋類APOBEC1の抗レトロウイルス・レトロエレメント活性の解析 (2012)
  • Author(s): 池田輝政、小糸厚
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 20121113-15
• [Presentation] Mamalian TREX-2 component GANP is involved in HIV-1 mRNA export (2012)
  • Author(s): Maeda K, Almofty SA, Singh SK, Eid MMA, Shimoda M, Ikeda T, Shida H, Koito A, Pham P, Goodman MF, and Sakaguchi N.
  • Organizer: 13th. Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 20121024-26
• [Presentation] APOBEC cytidine deaminase as a player of intrinsic immunity against HIV and retroelements (2012)
  • Author(s): Koito A, Ikeda T.
  • Organizer: BIT's 2nd Annual World Congress of Molecular & Cell Biology (CMCB)
  • Place of Presentation: Beijing, China
  • Year and Date: 20120518-20
  • Invited
• [Presentation] Intrinsic restriction activity by APOBEC enzymes against HIV and retroelement (2012)
  • Author(s): Koito A, Ikeda T.
  • Organizer: 4th International Conference on Drug Discovery & Therapy, Hot Topics in HIV Research
  • Place of Presentation: Dubai, UAE(教育講演)
  • Year and Date: 20120212-15
• [Presentation] キメラタンパク質を用いたAPOBEC1の抗HIV-1活性に関する責任部位の機能解析 (2011)
  • Author(s): 池田輝政、小糸厚
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京(東京)
  • Year and Date: 20111130-1202
• [Presentation] APOBEC3GのHIV-1粒子内への取り込みを制御する宿主因子GANP (2011)
  • Author(s): 前田和彦、Kumar SS、Ameen AS、Abbas EMM、池田輝政、小糸厚、坂口薫雄
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京(東京)
  • Year and Date: 20111130-1202
• [Presentation] Retrovirus, retroelement restrictions by APOBEC enzymes (2011)
  • Author(s): Koito A, Ikeda T.
  • Organizer: 12th. Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 20111019-21
• [Presentation] GANP promotes APOBEC3G encapsidation in virions to reduce viral infectivity (2011)
  • Author(s): Maeda K, Singh SK, Ameen AS, Eid MMA, Ikeda T, Koito A, Pham P, Goodman MF, Sakaguchi N.
  • Organizer: 12th. Kumamoto AIDS Seminar GCOE Joint International Symposium
  • Place of Presentation: Kumamoto, Japan
  • Year and Date: 20111019-21
• [Presentation] Intrinsic restriction activity by apoB mRNA editing enzyme APOBEC1 against the mobility of retroelements (2011)
  • Author(s): Koito A, Ikeda T.
  • Organizer: Frontiers of Retrovirology Conference, Complex retroviruses, retroelements and their hosts
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 20111003-05
• [Presentation] Identification of the functional region required for anti-HIV-1 activity of APOBEC1 (2011)
  • Author(s): Ikeda T, Koito A.
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110911-16
• [Presentation] Intrinsic restriction activity by APOBEC cytidine deaminases against the mobility of retroelements (2011)
  • Author(s): Koito A, Ikeda T.
  • Organizer: BIT's 1^<st> Annual World Congress of Molecular & Cell Biology (CMCB)
  • Place of Presentation: Beijing, China
  • Year and Date: 20110806-08
  • Invited
• [Presentation] GANP associated with HIV-1 RNA export is encapsidated with Apobec3G into virion cores (2011)
  • Author(s): Maeda K, Singh SK, Sarah A, Eid MM, Ikeda T, Pham P, Goodman MF, Koito A, Sakaguchi N.
  • Organizer: Cold Spring Harbor Laboratory, Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY USA
  • Year and Date: 20110523-28
• [Presentation] Identification of the functional region required for anti-HIV-1 activity of APOBEC1 (2011)
  • Author(s): Ikeda T, Koito A.
  • Organizer: Cold Spring Harbor Laboratory, Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, NY USA
  • Year and Date: 20110523-28
• [Presentation] シチジン脱アミノ化酵素によるレトロウイルス・レトロエレメントの制御 (2011)
  • Author(s): 小糸厚
  • Organizer: 同仁化学研究所フォーラム・イン・ドージン, RNAの識別と管理―自己、異常な自己、そして非自己
  • Place of Presentation: 熊本ホテルキャッスル(熊本)
  • Year and Date: 2011-11-04
  • Invited