The role of CCR5 oligomerization in the entry of CCR5-using HIV-1

2014 Fiscal Year Final Research Report

• Project/Area Number: 23590548
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Kumamoto University
• Principal Investigator: MAEDA Yosuke 熊本大学, 大学院生命科学研究部, 准教授 (30284764)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Kazuhisa 国立感染症研究所, エイズ研究センター, 室長 (60315306) ; HARADA Shinji 熊本大学, 大学院生命科学研究部, 教授 (60173085)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: HIV-1 / CCR5

Outline of Final Research Achievements

We found that CCR5 exists as constitutive homo-oligomers, which was further enhanced by its antagonists such as maraviroc (MVC). We further showed that that CCR5 oligomer was structurally different from the monomer. Infection of oligomer- and monomer-enriched cells revealed that CCR5-using HIV-1 preferential recognized monomeric CCR5. Although CCR5 antagonists enhanced oligomerization of CCR5, MVC-resistant HIV-1 was found to still recognize both MVC-bound and -unbound forms of monomeric CCR5, suggesting the constrained use of monomeric CCR5 by R5 HIV-1.

Free Research Field

ウイルス学