Pathogenesis of Saffold virus infection in animal models

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590554
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: NAGATA Noriyo 国立感染症研究所, 感染病理部, 室長 (30270648)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Hiroyuki 国立感染症研究所, ウイルス第二部, 室長 (90270644)
• Co-Investigator(Renkei-kenkyūsha): IWATA Naoko 国立感染症研究所, 感染病理部, 主任研究官 (10360695) ; SUZUKI Tadaki 国立感染症研究所, 感染病理部, 室長 (30527180)
• Research Collaborator: KOTANI Osamu 国立感染症研究所, 感染病理部, 研究生
• Project Period (FY): 2011 – 2013
• Keywords: 感染症 / ウイルス / 病理学 / 動物モデル / 病原性

Research Abstract

Saffold virus (SAFV) has been isolated and/or detected from human cases of diarrhea and upper respiratory tract inflammation in worldwide since the first report in 2007 and were considered epidemiologically that most people generally infected in early childhood. However, SAFV has been detected in the some cases of non-polio acute flaccid paralysis and a few cases of neuronal disorders. Then SAFV possibly be neurovirulent to human.
To understand the pathogenesis of SAFV, first of all, we established a detection system of virus antigen and virus genome in pathological diagnosis. Next, we evaluated the susceptibility of experimental animals to SAFV using two clinical isolates of SAFV. Then we attempted to generate an animal model of cerebellar hypoplasia after SAFV infection using neonatal mice.

Research Products

• [Journal Article] Transgenic mouse model for the study of enterovirus 71 neuropathogenesis (2013)
  • Author(s): Fujii K, Nagata N, Sato Y, Ong KC, Wong KT, Yamayoshi S, Shimanuki M, Shitara H, Taya C, Koike S
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Pages: 14753-14758
  • DOI: 10.1073/pnas.1217563110.
• [Journal Article] TNF-αacts as an immunoregulator in the mouse brain by reducing the incidence of severe disease following Japanese encephalitis virus infection (2013)
  • Author(s): Hayasaka D, Shirai K, Aoki K, Nagata N, Simantini DS, Kitaura K, Takamatsu Y, Gould E, Suzuki R, Morita K
  • Journal Title: PLoS One Pages: e71643
  • DOI: 10.1371/journal.pone.0071643
• [Journal Article] Enterovirus 71 binding to PSGL-1 on leukocytes : VP1-145 acts as a molecular switch to control receptor interaction (2013)
  • Author(s): Nishimura Y, Lee H, Hafenstein S, Kataoka C, Wakita T, Bergelson JM, Shimizu H
  • Journal Title: PLoS Pathog Pages: e1003511
  • DOI: 10.1371/journal.ppat.1003511
• [Journal Article] Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008 (2013)
  • Author(s): Sakai K, Nagata N, Ami Y, Seki F, Suzaki Y, Iwata-Yoshikawa N, Suzuki T, Fukushi S, Mizutani T, Yoshikawa T, Otsuki N, Kurane I, Komase K, Yamaguchi R, Hasegawa H, Saijo M, Takeda M, Morikawa S
  • Journal Title: J Virol Volume: 87 Pages: 1105-1114
  • DOI: 10.1128/JVI.02419-12
• [Journal Article] Susceptibility to flavivirus-specific antiviral response of Oas1b affects the neurovirulence of the Far- Eastern subtype of tick-borne encephalitis virus (2013)
  • Author(s): Yoshii K, Moritoh K, Nagata N, Yokozawa K, Sakai M, Sasaki N, Kariwa H, Agui T, Takashima. I
  • Journal Title: Arch Virol Volume: 158 Pages: 1039-1046
  • DOI: 10.1007/s00705-012-1579-1
• [Journal Article] Neuropathogenesis of a mouse-adapted porcine epidemic diarrhea virus infection in suckling mice (2012)
  • Author(s): Kotani O, Shirato K, Nagata N, Ikeda H, Takahashi K, Taguchi F
  • Journal Title: J Gen Virol Volume: 94 Pages: 831-836
  • DOI: 10.1099/vir.0.047787-0
• [Journal Article] Wild-type measles virus with the hemagglutinin protein of the edmonston vaccine strain retains wild-type tropism in macaques (2012)
  • Author(s): Takeuchi K, Nagata N, Kato SI, Ami Y, Suzaki Y, Suzuki T, Sato Y, Tsunetsugu-Yokota Y, Mori K, Van Nguyen N, Kimura H, Nagata K
  • Journal Title: J Virol Volume: 86 Pages: 3027-3037
  • DOI: 10.1128/JVI.06517-11
• [Journal Article] The toll-like receptor 3-mediated antiviral response is important for protection against poliovirus infection in poliovirus receptor transgenic mice (2012)
  • Author(s): Abe Y, Fujii K, Nagata N, Takeuchi O, Akira S, Oshiumi H, Matsumoto M, Seya T, Koike S
  • Journal Title: J Virol Volume: 86 Pages: 185-94
  • DOI: 10.1128/JVI.05245-11
• [Journal Article] Wild-Type Measles Virus is Intrinsically Dual-Tropic (2012)
  • Author(s): Takeda M, Tahara M, Nagata N, Seki F
  • Journal Title: Front Microbiol Volume: 2 Pages: 279
  • DOI: 10.3389/fmicb.2011.00279
• [Presentation] Comparative analyses of the pathogenicity of two isolates of Saffold virus in neonatal mouse (2014)
  • Author(s): Kotani O, Asif N, Suzuki T, Iwata N, Nakajima N, Katano H, Hosomi T, Tsukagoshi H, Hasegawa H, Taguchi F, Shimizu H, Nagata N
  • Organizer: Europic 2014
  • Place of Presentation: Belgium
  • Year and Date: 20140300
• [Presentation] A comparison of human enterovirus detection in experimentally infected neonatal mice using immunohistochemistry (2014)
  • Author(s): Nagata N, Kotani O, Iwata N, Suzuki T, Sato Y, Koike S, Iwasaki T, Taguchi F, Hasegawa H, Shimizu H
  • Organizer: Europic 2014
  • Place of Presentation: Belgium
  • Year and Date: 20140300
• [Presentation] コクサッキーウイルスB2実験室株脳内接種後のマウスにおける水頭症の発症機序 (2013)
  • Author(s): 潮田和佳、小谷治、岩田奈織子、鈴木忠樹、中島典子、長谷川秀樹、清水博之、永田典代
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20131100
• [Presentation] hSCARB2-Tgマウスを用いたEV71標的組織決定機構の解析 (2013)
  • Author(s): 藤井健、永田典代、小池智
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20131100
• [Presentation] エンテロウイルス71のカニクイザルにおける病原性の解析 (2013)
  • Author(s): 片岡周子、西村順裕、鈴木忠樹、小谷治、岩田奈織子、永田典代、網康至、清水博之
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20131100
• [Presentation] 新生仔マウスを用いたSaffold virus小脳継代株の作出とその病原性の解析 (2013)
  • Author(s): 小谷治、Naeem Asif、鈴木忠樹、岩田奈織子、中島典子、片野晴隆、長谷川秀樹、田口文広、清水博之、永田典代
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20131100
• [Presentation] 脳炎・髄膜炎関連ウイルスの病理学的検索のための参照標本の作製と抗体の検討 (2013)
  • Author(s): 永田典代、岩田奈織子、鈴木忠樹、佐藤由子、中島典子、片野晴隆、長谷川秀樹
  • Organizer: 第102回日本病理学会
  • Place of Presentation: 札幌
  • Year and Date: 20130400
• [Presentation] ヒトカルジオウイルス(Saffold virus)の新生仔マウス小脳における病理学的解析 (2013)
  • Author(s): 小谷治、鈴木忠樹、Naeem Asif、岩田奈織子、中島典子、片野晴隆、田口文広、長谷川秀樹、清水博之、永田典代
  • Organizer: 感染症若手フォーラム2013
  • Place of Presentation: 北海道
  • Year and Date: 20130200
• [Presentation] 新生仔マウスにおける新規ヒトカルジオウイルス(Saffold virus)の神経病原性の解析 (2012)
  • Author(s): 小谷治、鈴木忠樹、Naeem Asif、岩田奈織子、中島典子、片野晴隆、田口文広、長谷川秀樹、清水博之、永田典代
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
  • Year and Date: 20121100
• [Presentation] EV71感受性マウスモデルの作出と解析 (2012)
  • Author(s): 藤井健、永田典代、山吉誠也、島貫碧、設楽浩志、多屋長治、小池智
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
  • Year and Date: 20121100
• [Presentation] Pathological study of enterovirus 71 in animal models (2012)
  • Author(s): Nagata N.
  • Organizer: 第16回日本神経ウイルス研究会シンポジウムCurrent Progress in Enterovirus 71 Research in the Asia - Pacific Region
  • Place of Presentation: 東京
  • Year and Date: 20120800
• [Presentation] Neuropathogenesis of mouse-adapted porcine epidemic virus infection in suckling mouse (2011)
  • Author(s): Kotani O, Shirato K, Nagata N, Miyazaki A, Ikeda H, Taguchi F, Takahashi K
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 20110900
• [Book] ウイルス感染症の検査・診断スタンダード, 第11章電子顕微鏡/病理組織学的検査
  • Author(s): 永田典代、長谷川秀樹、佐多徹太郎, (田代眞人、牛島廣治編)
  • Total Pages: 410-439
  • Publisher: 羊土社
• [Book] ウイルス感染症の検査・診断スタンダード, 第2章ウイルス分離培養第3項実験動物、等
  • Author(s): 永田典代、長谷川秀樹, (田代眞人、牛島廣治編)
  • Total Pages: 257-268
  • Publisher: 羊土社