2013 Fiscal Year Final Research Report
Study of human norovirus replication and pathogenicity
| Project/Area Number |
23590556
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 病原性 / 細胞内同多 / 機能 |
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| Research Abstract |
We established a new reverse genetics system driven by a mammalian promoter, that functions without helper virus. The complete genome of the HuNoV GII.3 U201, Saga1, TCH, NV68 and also MNV-S7 strain was cloned downstream of an EF-1 alpha mammalian promoter. These constructs produced progeny viruses produced from cells that contained the complete NoV genomic RNA, VP1, VP2 and VPg were detected in isopycnic gradients of CsCl at the same density as native infectious NoV particles from a patient's stool. A GFP reporter construct containing the GFP gene in ORF1 produced complete virions that contain VPg linked RNA. RNA from virions containing the encapsidated GFP genomic RNA was successfully transfected back into cells producing fluorescent puncta indicating that the encapsidated RNA is replication competent. Our results are useful to develop of an antiviral medicine which targeted these in future.
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[Journal Article] Norovirus binding to intestinal epithelial cells is independent of histo-blood group antigens2013
Author(s)
Murakami K, Kurihara C, Oka T, Shimoike T, Fujii Y, Takai-Todaka R, Park Y, Wakita T, Matsuda T, Hokari R, Miura S, Katayama K
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Journal Title
PLoS One
Volume: 8(6), (Print)
Pages: e66534
DOI
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[Presentation] Selective constraints on changes of a norovirus pandemic lineage GII.4_2006b2013
Author(s)
Sato H, Yokoyama M, Motomura K, Nakamura H, Oka T, Katayama K, Takeda N, Noda M, Tanaka T, and the Norovirus Surveillance Group of Japan
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China
Year and Date
20131012-15
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[Presentation] Deep Sequencing-based analysis of minor variants in norovirus infection cases with acute gastroenteritis2013
Author(s)
Motomura K, Ode H, Yokoyama M, Nakamura H, Sato A, Katayama K, Noda M, Takeda N, Tanaka T, Sato H and the Norovirus Surveillance Group of Japan
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China
Year and Date
20131012-15
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[Presentation] Estes^1 A plasmid based reverse genetics system can drive human and murine norovirus genome replication and produce progeny virus containing reporter tagged infectious genomic RNA2013
Author(s)
Kazuhiko Katayama^<1,2>, Reiko Takai-Todaka, Akira Nakanishi, Kosuke Murakami^<1,2>, Tomoichiro Oka, Susana Guix, Tyler M. Sharp, Robert L. Atmar , Sue E. Crawford, and Mary K
Organizer
The Fifth International Calicivirus Conference
Place of Presentation
Beijing, China
Year and Date
20131012-15
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