2013 Fiscal Year Final Research Report
Preparation of sialylated IgG and elucidation of the mechanism for its anti-inflammatory properties
| Project/Area Number |
23590578
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | National Hospital Organization,Yamaguchi - Ube Medical Center |
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Principal Investigator |
MIMURA YUSUKE 独立行政法人国立病院機構山口宇部医療センター(臨床研究部), その他部局等, 研究員 (00219718)
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| Project Period (FY) |
2011 – 2013
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| Keywords | IgG / glycosylation / sialylation / DC-SIGN |
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| Research Abstract |
The attachment of oligosaccharide, at Asn297, of the IgG-Fc heavy chain is essential for optimal activation of Fc effector functions. It has recently been shown that IgG with oligosaccharides bearing terminal sialic acid residues mediates anti-inflammatory therapeutic effects. We expressed an IgG1 Phe243Ala (F243A) mutant in both human and rodent cell lines, and sialylation was found to be markedly increased. The mechanism by which the sialic acid residues in the IgG-Fc oligosaccharides exert an anti-inflammatory effect has been investigated by using dendritic cells that express DC-SIGN.
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