2013 Fiscal Year Final Research Report
Tests for quantity and characteristics of acute leukemia stem cells to search molecular-targeted therapies suitable for each case
| Project/Area Number |
23590662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SHUJI TOHDA 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (80251510)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 急性白血病 / 白血病幹細胞 / 分子標的治療 / 薬剤感受性検査 / Notch |
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| Research Abstract |
To investigate the roles of stemness-related signals in the growth of leukemia cells, the signals were activated by the ligand-stimulation and were suppressed by adding the inhibitors or siRNA (small interfering RNA) in culture systems of leukemia cells. Activation of Hedgehog signal did not affect the short-term growth while it promoted the self-renewal of clonogenic cells. Activation of BMP (Bone Morphogenetic Protein) signal promoted the growth of some samples while it suppressed the colony formation. Suppression of Notch, Hedgehog, Wnt, mTOR (mammalian Target of Rapamycin), and HIF (Hypoxia Inducible Factor) signals by adding each inhibitor or siRNA suppressed the growth. We found the cross-talk between these signals. The analysis of Notch expression by flow cytometry could not quantitate the leukemia stem cells.
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