Establishment of plasma biomarker for Alzheimer's disease and mild cognitive impairment through a longitudinal follow-up study

2015 Fiscal Year Final Research Report

• Project/Area Number: 23590669
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Doshisha University (2012-2015); Kyoto University (2011)
• Principal Investigator: Matsumoto Akira 同志社大学, 研究開発推進機構, 嘱託研究員 (80181759)
• Co-Investigator(Kenkyū-buntansha): TAKECHI Hajime 京都大学, 医学部, 講師 (10314197)
• Project Period (FY): 2011-04-28 – 2016-03-31
• Keywords: 軽度認知障害 / アルツハイマー病 / バイオマーカー / 縦断的臨床研究 / エンドプロテアーゼ / 補体 / 立体構造解析

Outline of Final Research Achievements

In order to find biomarker candidates that can discriminate subjects with mild cognitive impairment (MCI) among normal elderly, we have adopted a longitudinal follow-up study of clinical and lavoratory information and plasma sampling. Basically, comparative study of plasma samples derived from the same subject but different clinical and laboratory conditions was carried out. Within four years of clinical follow-up, we have identified six cases which developped from normal cognitive condition to MCI.
Using mass-spectrometrical analyses, one peptide candidate was found in plasma samples derived from MCI stage but not from normal stage of the all six cases. The candidate peptide which is uniquely expressed in MCI plasma have been identified as an endoproteinase cleavage pruduct of a compliment protein expressed in plasma. A novel assay system, employing a monoclonal antibody which recognizes three-dimentional structure of the C-terminalcleavage site is now under progress.

Free Research Field

病態生化学