2013 Fiscal Year Final Research Report
Establishment of FACS-mQ as a tool for detecting stem cells or cancer stem cells
| Project/Area Number |
23590670
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Osaka University |
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Principal Investigator |
TAKANO Toru 大阪大学, 医学(系)研究科(研究院), 講師 (00263236)
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| Project Period (FY) |
2011 – 2013
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| Keywords | FACS-mQ / flow cytometry / 幹細胞 / 癌幹細胞 / RNA / 遺伝子発現プロフィール |
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| Research Abstract |
The basic protocols of FACS-mQ(mRNA quantification after FACS) were established. In FACS-mQ, intracellular RNAs are preserved without degradation after immunolabeling with a florophore, then we can analyze gene expression profiles of sorted cells after FACS. We established the following three protocols: 1)preparation of single cells from blood or tissue samples without RNA degradation, 2)high sensitive immunolabeling of the targeted cells, and 3) recovery and analysis of a samll amount of RNAs from sorted cells.
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[Journal Article] Genes that characterize T3-predominant Graves' thyroid tissues2013
Author(s)
Matsumoto, C., Ito, M., Yamada, H., Yamakawa, N., Yoshida, H., Date, A., Watanabe, M., Hidaka, Y., Iwatani, Y., Miyauchi, A., Takano, T
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Journal Title
Eur J Endocrinol
Volume: 168
Pages: 137-144
DOI
Peer Reviewed
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[Journal Article] Preparation of thyroid follicular cells for mRNA quantification after fluorescence-activated cell sorting2013
Author(s)
Matsumoto, C., Ito, M., Yamada, H., Yoshida, H., Watanabe, M., Hidaka, Y., Iwatani, Y., Miyauchi, A., Takano, T
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Journal Title
Scand J Clin Lab Invest
Volume: 173
Pages: 245-252
DOI
Peer Reviewed
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[Journal Article] Prolonged hybridization with a cRNA probe improves the signal to noise ratio for in-tube in situ hybridization for quantification of mRNA after fluorescence-activated cell sorting2012
Author(s)
Yamada, H., Yamakawa, N., Watanabe, M., Hidaka, Y., Iwatani, Y., Takano, T
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Journal Title
Biotech Histochem
Volume: 87
Pages: 366-371
DOI
Peer Reviewed
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[Journal Article] Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration : The first clinical trial in 130 cases2012
Author(s)
Yamada, H., Takano, T., Kihara, M., Hirokaw,a M., Yoshida, H., Watanabe, M., Iwatani, Y., Hidaka, Y., .Miyauchi, A
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Journal Title
Endocr J
Volume: 59(JST.JSTAGE/endocrj/EJ12-0077 [pii])
Pages: 621-630
Peer Reviewed
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[Journal Article] mRNA quantification after fluorescence activated cell sorting using locked nucleic Acid probes2011
Author(s)
Maruo, R.,Yamada, H., Watanabe, M., Hidaka, Y., Iwatani, Y., and Takano, T
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Journal Title
Mol Biotechnol
Volume: 49
Pages: 42-47
DOI
Peer Reviewed
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[Journal Article] An improved protocol for mRNA quantification after fluorescence activated cell sorting with an increased signal to noise ratio in flow cytometry
Author(s)
Date, A., Maeda, T., Watanabe, M., Hidaka, Y., Iwatani, Y., Takano, T
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Journal Title
Mol Biotechnol
Volume: (in press)
DOI
Peer Reviewed
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