2013 Fiscal Year Final Research Report
New method of cat scratch disease by ELISA using specific antigens and its application to the pathological clarification
| Project/Area Number |
23590673
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TSUNEOKA Hidehiro 山口大学, 医学(系)研究科(研究院), 教授 (40437629)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIHARA Kiyoshi 山口大学, 大学院医学系研究科, 教授 (10144495)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 猫ひっかき病 / ＥＬＩＳＡ |
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| Research Abstract |
The measurement of Bartonella henselae IgG by highly-sensitive ELISA requires highly-specific purification of antigen. The extract of B. henselae ATCC49882 by N-lauroyl sarcosinate includes a great deal of useful protein component. We directly extracted B. henselae ATCC49882 by N-lauroyl sarcosinate for 2-4 times without supersonic treatment and purified it by diethlaminoethyl ion-exchange (DEAE) chromatography. As a result, it was clarified that highly-specific protein components were eluted with the salt concentration of 245-295mM. Further development of ELISA and its availability by using this antigenic component will be greatly expected.
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