2013 Fiscal Year Final Research Report

Development and clinical application of the immunochromatography for a citrin protein

Research Project

Project/Area Number	23590680
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Laboratory medicine
Research Institution	Kagoshima University
Principal Investigator	IIJIMA Mikio 鹿児島大学, 医歯(薬)学総合研究科, 助教 (00305111)
Project Period (FY)	2011 – 2013
Keywords	イムノクロマト法 / ミトコンドリア / アスパラギン酸・グルタミン酸輸送体
Research Abstract	Citrin deficiency causes neonatal intrahepatic cholestasis caused by citrin deficiency, and adult-onset type II citrullinemia. Diagonosis of citrin deficiency is mainly performed by genetic analysis, although the known mutations are not detected in some cases. Western blot analysis is also performed for citrin deficiency. The use of Western blot analysis is very few because it is time-consuming. Immunochromatography is recently developed detection method, which is easy and quick. The aim of this project is to examine citrin protein in oral mucosae by using immunochromatography as an alternative diagnostic method. In this project, the development of immunochromatography for a citrin protein was not completed.