Identification of novel gastric cancer risk markers

2014 Fiscal Year Final Research Report

• Project/Area Number: 23590682
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: MAEKITA TAKAO 和歌山県立医科大学, 医学部, 講師 (10326358)
• Co-Investigator(Kenkyū-buntansha): ICHINOSE Masao 和歌山県立医科大学, 医学部, 教授 (50143425)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: エピジェネティクス / 胃癌 / リスクマーカー

Outline of Final Research Achievements

This study aimed to elucidate the correlation between altered mucosal DNA methylation levels and activity of H. pylori-related gastritis, because inflammatory activity are associated with the development of diffuse-type gastric cancer. Methylation levels (promoter CpG islands (FLNc,HAND1, THBD, p41ARC, HRASLS, and LOX) and the CpG sites of non-coding repetitive elements (Alu and Sata) were examined by real-time methylation-specific PCR or bisulfite pyrosequencing. Methylation levels of the six CpG islands were consistently increased, and those of the two repetitive elements were consistently decreased in a stepwise manner with the activity of gastric inflammation. The observed correlation between altered DNA methylation levels and activity of H. pylori-related gastritis appears to be one of the relevant molecular mechanisms underlying the development of diffuse-type cancer.
We might identify novel diffuse-type gastric cancer risk marker.

Free Research Field

エピジェネティクス