Analysis of ATBF1 expression in malignant mesothelioma with reference to its intracellular behavior and association with cancer stem cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590692
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kanazawa Medical University
• Principal Investigator: MINATO Hiroshi 金沢医科大学, 医学部, 教授 (10293367)
• Co-Investigator(Kenkyū-buntansha): MIURA Yutaka 名古屋市立大学, 医学(系)研究科(研究 院), 准教授 (90285198) ; NAKADA Satoko 金沢医科大学, 医学部, 助教 (30569091) ; TAKEGAMI Tsutomu 金沢医科大学, 総合医学研究所, 教授 (10113490)
• Project Period (FY): 2011 – 2013
• Keywords: 悪性中皮腫 / 癌抑制遺伝子 / ATBF1 / 癌幹細胞 / 免疫組織化学 / 細胞内局在

Research Abstract

We analyzed expression of ATBF1 protein in malignant mesothelioma and reactive mesothelial cells. Using anti-D1-120 antibody, ATBF1 expressed less frequently in the nuclei of malignant mesotheliomas, and significantly increased its expression in the cytoplasm. The cytoplasmic expression significantly correlated with the prognosis of malignant mesothelioma. Using anti-MB043 antibody, ATBF1 expressed with large dots in the nuclei of malignant mesothelial cells, whereas did not stained in the reactive mesothelial cells, thus MB043 is considered to be a useful marker for differentiating between malignant and benign mesothelial cells. The results indicate that expression of ATBF1 increases in malignant mesothelioma, but its localization probably changes in the tumor cells. ATBF1 may exist through the nucleus to the cytoplasm, or may be segmentized in the nucleus and the cytoplasm.

Research Products

• [Journal Article] Comparative immunohistochemical analysis of IMP3, GLUT1, EMA, CD146, and desmin for distinguishing malignant mesothelioma from reactive mesothelial cells (2014)
  • Author(s): Minato H, Kurose N, Fukushima M, Nojima T, Usuda K, Sagawa M, Sakuma T, Ooi A, Matsumoto I, Oda M, Arano Y, Shimizu J
  • Journal Title: Am J Clin Pathol Volume: 141 Pages: 85-93
  • DOI: 10.1309/AJCP5KNL7QTELLYI
  • Peer Reviewed
• [Journal Article] AT motif binding factor 1 (ATBF1) is highly phosphorylated in embryonic brain and protected from cleavage by calpain-1 (2012)
  • Author(s): Zhang S, Kim TS, Dong Y, Kanazawa S, Kawaguchi M, Gao N, Minato H, Takegami T, Nojima T, Miura Y
  • Journal Title: Biochem Biophys Res Commun Volume: 427 Pages: 537-541
  • DOI: 10.1016/j.bbrc.2012.09.092
  • Peer Reviewed
• [Presentation] 反応性中皮と悪性中皮腫におけるATBF1の核細胞質発現:免疫組織化学的検討 (2013)
  • Author(s): 湊宏, 川口誠, 三浦裕, 張勝, 中田聡子, 他
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
  • Year and Date: 20130606-08
• [Presentation] Comparative immunohistochemical analysis to distinguish malignant mesothelioma (MM) from reactive mesothelial cells (RMC) (2012)
  • Author(s): Minato H, Fukushima H, Kurose N, Nojima T
  • Organizer: 101th Annual meeting of the United States and Canadian Academy of Pathology
  • Place of Presentation: バンクーバー,カナダ
  • Year and Date: 20120317-23
• [Presentation] ATBF1 is highly phosphorylated in embryonic brain (2011)
  • Author(s): Zhang S, Dong Y, Minato H, Nojima T, Asai K, Miura Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] ATBF1の細胞内局在の悪性中皮腫の再評価 (2011)
  • Author(s): 湊宏, 他
  • Organizer: 第4回ATBF1研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-11-12
• [Remarks]
  • URL: http://www.kanazawa-med.ac.jp/~clinpath/g1_study/index.html