2013 Fiscal Year Final Research Report
Approach to the molecular mechanisms of hematopoietic dysplasia aiming at elucidation of the pathogenesis of myelodysplastic syndromes
| Project/Area Number |
23590696
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
TOHYAMA Kaoru 川崎医科大学, 医学部, 教授 (80227561)
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| Co-Investigator(Renkei-kenkyūsha) |
TASAKA Taizo 川崎医科大学, 医学部, 准教授 (80343309)
TSUJIOKA Takayuki 川崎医科大学, 医学部, 講師 (50330551)
SUEMORI Shin-ichiro 川崎医科大学, 医学部, 講師 (60330552)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 遺伝子ノックダウン法 / 血球異形成 / 食細胞機能 |
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| Research Abstract |
I focused attention on vimentin, one of the intermediate filaments which support cell shape and might be involved in the mechanisms of hematopoietic dysplasia, and examined the change of vimentin expression during the differentiation process of human leukemic HL60 cell line. Vimentin expression was down-regulated in the process of neutrophil-like differentiation of HL60 cells, while it was extremely up-regulated in the process of macrophage-like differentiation.
Next, I established HL60 cell clones whose vimentin expression was knock-downed by transfer of a lentiviral vector harboring vimentin-shRNA. Such vimentin-knock-downed clones were induced to differentiation and revealed increased leukocyte activity such as cell movement and phagocytosis. These data suggested that vimentin regulates leukocyte function negatively.
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[Journal Article] Rab27b regulates c-kit expression by controling the secretion of stem cell factor2012
Author(s)
Tanaka C, Kaji H, He J, Hazama R, Yokoyama K, Kinoshita E, Tsujioka T, Tohyama K, Yamamura H, Nishio H, Tohyama Y
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Journal Title
Biochem Biophys Res Comm
Volume: 419
Pages: 368-373
DOI
Peer Reviewed
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[Journal Article] Morphologic analysis in myelodysplastic syndromes with del(5q) treated with lenalidomide. A Japanese multiinstitutional study2012
Author(s)
Matsuda A, Taniwaki M, Jinnai I, Harada H, Watanabe M, Suzuki K, Yanagita S, Suzuki T, Yoshida Y, Kimura A, Tsudo M, Tohyama K, Takatoku M, Ozawa K
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Journal Title
Leuk Res
Volume: 36
Pages: 575-580
DOI
Peer Reviewed
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[Journal Article] Clinical utility of the neutrophil distribution pattern obtained using the CELL-DYN SAPPHIRE hematology analyzer for the diagnosis of myelodysplastic syndrome2011
Author(s)
Inaba T, Yuki Y, Yuasa S, Fujita N, Yoshitomi K, Kamisako T, Torii K, Okada T, Urasaki Y, Ueda T, Tohyama K
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Journal Title
Int J Hematol
Volume: 94
Pages: 169-77
DOI
Peer Reviewed
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[Journal Article] Genome-wide identification of human microRNAs located in leukemia-associated genomic alterations2011
Author(s)
Starczynowski DT, Morin R, McPherson A, Lam J, Chari R, Wegrzyn J, Kuchenbauer F, Hirst M, Tohyama K, Humphries RK, Lam WL, Marra M, Karsan A
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Journal Title
Blood
Volume: 117
Pages: 595-607
DOI
Peer Reviewed
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[Journal Article] 骨髄増殖性腫瘍におけるJAK2-V617F変異遺伝子比率と好中球アルカリホスファターゼスコアとの関連2011
Author(s)
奥野菜々子, 近藤敏範, 岸本光代, 辻岡貴之, 末盛晋一郎, 松岡亮仁, 久山亜紀, 田坂大象, 大倉貢, 野田幸代, 通山薫
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Journal Title
日本検査血液学会雑誌
Volume: 12
Pages: 170-176
Peer Reviewed
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