2014 Fiscal Year Final Research Report
Research of established monoclonal antibodies against tumor related molecules in a pancreatic juice
| Project/Area Number |
23590698
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Research Collaborator |
YANO Hirohisa 久留米大学, 医学部, 教授
OHSHIMA Kouichi 久留米大学, 医学部, 教授
YASUMOTO Makiko 久留米大学, 医学部・大学院
NISHINAKAGAWA Takuya 福岡大学, 薬学部, 助教
HAMABASHIRI Masato 福岡大学, 大学院
DAICHO Aya 福岡大学, 大学院
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | 免疫学 / 癌マーカー / 単クローナル抗体 / 膵癌 / 膵液 / Tn 抗原 / シアリル化ルイス a 抗原 |
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| Outline of Final Research Achievements |
We have established mouse monoclonal antibodies (2P-1-2-1, 2P-1-17-1, 6P-3-2-4) against malignant tumor related molecules in a pancreatic juice. The molecules recognized by these monoclonal antibodies were studied. Because 2P-1-17-1 and 6P-3-2-4 have shown immune-reactivity against glycol-antigen resulted by western-blotting analysis, synthetic glycol-pepetides were used as antigens for ELISA and Westernblotting assay. As a consequence of these assay, 2P-1-17-1 and 6P-3-2-4 reacted with sialyl lews a and Tn antigen, respectively. 2P-1-2-1 recognized the molecule which was 100~130 kDa in molecular size with a fine band on westernblotting. The molecule was purified by immune-precipitation method using protein G coated magnetic beads and isolated by SDS-PAGE gel electrophoresis. Isolated molecule in SDS-PAGE gel was analysed by LC-MS/MS, resulting that molecule was a bile salt-dependent lipase oncofetal isoform, which was specifically produced in pancreas acinal cell, with sore 828.
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| Free Research Field |
がん免疫
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