2013 Fiscal Year Final Research Report
A role of purinergic receptor on descending pain modulatory system in anterior cingulate cortex of chronic pain
| Project/Area Number |
23590706
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Hirosaki University |
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Principal Investigator |
MIGITA KEISUKE 弘前大学, 医学(系)研究科(研究院), 助教 (10352262)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Shinya 弘前大学, 大学院医学研究科, 教授 (00312158)
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| Co-Investigator(Renkei-kenkyūsha) |
HONDA Kenji 福岡大学, 薬学部, 助教 (60140761)
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| Project Period (FY) |
2011-04-28 – 2014-03-31
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| Keywords | 慢性疼痛 / 中枢神経系 / IPSC / プリン受容体 |
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| Research Abstract |
In this study, we investigated the phenotypes of pain behaviors in phospholipase C-related inactive protein type 1 knockout (PRIP-1-/-) mice that play an important role in trafficking to the plasma membrane of GABAA receptor. PRIP-1-/- mice showed the hyperalgesic responses induced by formalin and von Frey filaments. In situ hybridization studies of GABAA receptors revealed that expression of gamma 2 subunit mRNA was significantly decreased in the dorsal and ventral horn of spinal cord of PRIP-1-/- mice, whereas beta 2 subunit mRNA expression was significantly increased in all areas of the spinal cord. Patch-clamp recordings showed that the function of GABAA receptor in spinal cord was impaired by knockout of PRIP-1. On the other hand, HSP90 regulates the P2X7 current by interacting with the cysteine-rich domain of the cytoplasmic C-terminus. PRIP-1 and P2X7 receptor may become a new drug target for neuropathic pain.
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Research Products
(16 results)
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[Journal Article] The kick-in system : a novel rapid knock-in strategy2014
Author(s)
Tomonoh Y, Deshimaru M, Araki K, Miyazaki Y, Arasaki T, Tanaka Y, Kitamura H, Mori F, Wakabayashi K, Yamashita S, Saito R, Itoh M, Uchida T, Yamada J, Migita K, Ueno S, Kitaura H, Kakita A, Lossin C, Takano Y, Hirose S
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Journal Title
PLoS One
Volume: 9
Pages: e88549
DOI
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[Journal Article] Morphologic changes of dendritic spines of striatal neurons in the levodopa-induced dyskinesia model2014
Author(s)
Nishijima H, Suzuki S, Kon T, Funamizu Y, Ueno T, Haga R, Suzuki C, Arai A, Kimura T, Suzuki C, Meguro R, Miki Y, Yamada J, Migita K, Ichinohe N, Ueno S, Baba M, Tomiyama M
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Journal Title
Mov Disord
Volume: 29
Pages: 336-343
DOI
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[Journal Article] A novel prophylactic effect of furosemide treatment on autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE)2013
Author(s)
Yamada J, Zhu G, Okada M, Hirose S, Yoshida S, Shiba Y, Migita K, Mori F, Sugawara T, Chen L, Liu F, Yoshida S, Ueno S, Kaneko S
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Journal Title
Epilepsy Res
Volume: 107
Pages: 127-137
DOI
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[Journal Article] Dysfunction of extrasynaptic GABAergic transmission in phospholipase C-related, but catalytically inactive protein 1 knockout mice is associated with an epilepsy phenotype2012
Author(s)
Zhu G, Yoshida S, Migita K, Yamada J, Mori F, Tomiyama M, Wakabayashi K, Kanematsu T, Hirata M, Kaneko S, Ueno S, Okada M
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Journal Title
J Pharmacol Exp Ther
Volume: 340(3)
Pages: 520-528
DOI
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[Presentation] GABA_A receptor via phospholipase C-related but catalytically inactive protein type 1 in the spinal cord participate in abnormal pain sensation2012
Author(s)
Keisuke Migita, Masahiko Tomiyama, Junko Yamada, Yoshikazu Nikaido, Takashi Kanematsu, Masato Hirata, Shinya Ueno Yamada, Yoshikazu Nikaido, Takashi Kanematsu, Masato Hirata, Shinya Ueno
Organizer
Neuroscience2012
Place of Presentation
New Orleans, USA
Year and Date
2012-10-13
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